Relevance. Early diagnosis of chronic overstress among athletes remains an important problem for coaches and specialists in the field of sports physiology and medicine. The goal is to study in an animal model the dynamics of autoimmune response to physical activity of different duration and intensity and to establish the prospects of the method of determining autoantibodies to cardiomyocyte proteins as an indicator of the morphofunctional state of the heart in the conditions of adaptation to muscle loads. Materials and Methods. The study was conducted in male white rats. Animals were subjected to 9 weeks of training simulated with treadban. The intensity of the load changed the angle of inclination and the speed of the tape. The amount of cardiospecific autoantibodies (auto-AB) to troponin I, to alpha-actin 1, to the human cardiac beta-myosin heavy chain MYH7 was determined in the blood by enzyme immunoassay. The relative heart mass was measured. Histomorphological assessment of cardiomyocyte condition was carried out. Statistical processing was carried out using the Student and Mann-Whitney criteria. Results and Discussion. Animal training was accompanied by moderate cardiac hypertrophy of pathological changes in cardiomyocytes. Heart weight increased by 6.9 %; 10.6 %; 12.9 % in the dynamics of 6-8-9 weeks of training. Concentrations of auto-AB to troponin I and to alpha-actin 1 were characterized by cyclicity, manifested by an increase in week 2 and a decrease by the 8th and 9th weeks of training. In the dynamics of 0-2-8-9 weeks of the experiment, the amount of auto-AB to troponin I was: 3.10.3; 4.20.9; 2.10.2; 2.00.04 ng/ml. For auto-AB to actin: 26.71.2; 31.31.4; 13.71.8; 12.11.6 ng/ml, respectively. The level of auto-AB to beta-myosin was manifested by a decrease in the dynamics of 0-6-9 weeks of training and amounted to: 16.30.9; 10.91.5; 8.20.8; 9.6 0.9 ng/ml. Conclusion. The results of determining cardiospecific auto-AB demonstrate a clear response of the immune system to the processes taking place in cardiomyocytes, which makes it possible to recommend further study of the method of determining auto-AB to cardiomyocyte proteins as a diagnostic test of the functional state of the heart muscle during the period of adaptation to physical activity.

Internal diseases 202ВНУТРИВЕННОЕ ЛАЗЕРНОЕ ОБЛУЧЕНИЕ КРОВИ пРИ РЕВмАТОИДНОм АРТРИТЕ: ДИНАмИКА пОКАЗАТЕЛЕй цИТОКИНОВОГО пРОФИЛЯ И АКТИВНОСТИ ЗАБОЛЕВАНИЯ Н. Н. БУРДУЛИ, Н. М. БУРДУЛИ Изучена динамика показателей цитокиново-го профиля у больных ревматоидным артритом при включении в терапию внутривенного лазер-ного облучения крови. Обследовано 102 паци-ента. Диагноз устанавливали на основании кли-нических, лабораторных и инструментальных методов исследования, в соответствии с крите-риями ACR/EULAR.Полученные данные свидетельствуют о на-личии дисбаланса в цитокиновой сети у больных ревматоидным артритом с преобладанием се-креции провоспалительных цитокинов.Комплексная терапия с применением ла-зерной терапии сопровождалась устранением дисбаланса в цитокиновой сети, приводила к более раннему и быстрому снижению активно-сти ревматоидного артрита и способствовала наступлению ремиссии у большего числа па-циентов, чем традиционная медикаментозная терапия.Ключевые слова: ревматоидный артрит, противовоспалительные, провоспалительные цитокины, внутривенное лазерное облучение крови The dynamics of cytokine profile in patients with rheumatoid arthritis treated with intravenous laser radiation was studied.102 patients were examined using clinical, laboratory and instrumental methods in accordance with the criteria ACR/EULAR for rheumatoid arthritis.The data obtained demonstrated the imbalance of the cytokine profile in rheumatoid arthritis patients with the predominance of the proinflammatory cytokines secretion.Laser therapy contributed to the improving of the cytokine network balance, led to earlier decrease of rheumatoid arthritis activity and resulted in faster remission in comparison with the traditional drug therapy.Key words: rheumatoid arthritis, antiinflammatory, proinflammatory cytokines, intravenous laser radiation © Коллектив авторов, 2014 УДК 796.015, 615.375.

Северо-кавказский федеральный университет, Ставрополь, Российская Федерация 2 Ставропольский государственный аграрный университет, Российская Федерация ammonium gas effecT on The male raTs' reproducTiVe sYsTem Belyaev n. g. 1 , Timchenko l. d. 1 , rzhepakovsky i. V. 1 , piskov s. i. 1 , grudneva a. d. 1 , mikhaylenko V. V. 2 , nagdalian a. a. 1 1 north caucasus federal university, stavropol, russian federation 2 stavropol state agrarian university, russian federation

Bone mineral densiTY of female raTs in The process of adapTaTion To simulaTed duraTiVe inTermiTTenT hYpoBaric hYpoXia Belyaev n. g., rzhepakovsky i. V., piskov s. i., plyuiko V. V. north-caucasus federal university, stavropol, russian federation Представлена оценка минеральной плотности бедренной кости методом рентгеновской компьютерной микротомографии и показателей адаптивных процессов самок крыс при моделировании хронической прерывистой гипобарической гипоксии. Увеличение массы сердца и надпочечников, повышение количества эритроцитов и гемоглобина явились маркерами компенсаторных процессов. Полученные результаты указывают на то, что длительное прерывистое воздействие гипобарической гипоксии как в условиях жесткой адаптации, так и с постепенным наращиванием стресс-фактора служит фактором, способствующим остеопоротическим изменениям в костной ткани. Ключевые слова: гипоксическая гипоксия, гипобарическая хроническая гипоксия, минеральная плотность костной ткани, самки крыс, адаптация The estimation of femoral mineral density by Х-ray computer microtomography and indicators of adaptive processes in female rats in the simulation of chronic intermittent hypobaric hypoxia is presented. An increase in heart and adrenal mass, an increase in the number of red blood cells and hemoglobin were markers of compensatory processes. The obtained results indicate that long-term intermittent exposure to hypobaric hypoxia, both in conditions of severe adaptation, and with a gradual build-up of the stress factor, can lead to osteoporotic changes in the bone tissue.

Relevance. In conditions of ever-increasing volume of training loads, the frequency of cases of chronic physical overstrain (CPO) among athletes is increasing. It determines the importance of early diagnosis of the formed pathology of the cardiovascular system in order to prevent its further development. The aim of the study was to study the dynamics of autoantibodies to cardiomyocyte proteins using an experimental model of CPO and to determine the prospects of a laboratory method for the determination of autoantibodies for early diagnosis of pathomorphological changes in the heart. Materials and Methods. The study was conducted on male white rats. A treadmill was used to model CPO. In animals, the heart rate was measured, electrical phenomena in the heart were recorded. The content of hemoglobin and erythrocytes was determined in the blood. The level of cardiospecific autoantibodies (auto-AB) to troponin I, to alpha-actin 1, and to the heavy chain of beta-myosin 7B was measured. Heart mass was measured and histomorphological assessment of the state of cardiomyocytes was carried out. Results and Discussion. While modeling CPO, a decrease in body weight of the animals, the development of anemia, and cardiac hypertrophy were recorded. A decrease in body weight by more than 30 % was recorded from days 25 to 35 of the modeled CPO. A decrease in the number of erythrocytes in the blood was noted on day 25 with a peak fall on days 30-35. The mass of heart of animals in the dynamics of 0-15-35 days was 0.39±0.003; 0.41±0.001; 0.44±0.005 g/100 g, respectively. On day 25, sinus tachycardia was recorded in 2 % of the animals. On days 30 and 35, in 10 % of the studied rats, a violation of the processes of repolarization of the left ventricle by the type of subepicardial ischemia was recorded. On the 25th day, fibrosis of the perivascular region was visualized, passing into the interstitial field between the myofibrils. Reticulate structures of connective tissue fibers between cardiomyocytes were found. The period of 30-35 days was characterized by even greater severity of pathomorphological changes: myocardial hypertrophy, moderate myocardial dystrophy, interstitial and perivascular fibrosis. An increase in the number of detectable auto-ABs to cardiomyocyte proteins was noted on the 10th day of the experiment. A multiple increase in autoantibodies to cardiomyocyte proteins was recorded earlier than functional disorders in the heart and morphological changes in cardiomyocytes were detected. Conclusion. The laboratory method for determining auto-ABs to myocardial proteins can be the earliest of the complex methods for diagnosing disorders that are formed in the body in conditions of adaptation to intense and prolonged physical exertion.