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3D bioprinting is the hi-tech approach in tissue engineering science. Applying of modern 3D bioprinting systems allows production of tissue-like structures that could be used in regenerative medicine. Such tissues or primitive organ constructs could be used for substituting the parts of damaged organs, or for changing of organs in whole. Biomaterials must fulfill specific requirement to be applied in regenerative medicine: biocompatibility, biodegradability and possessing appropriate mechanical properties. PEG and PCL are widely used today in tissue engineering. We optimized wide range of PCL, PEG solutions alone and in combination. For 3D bioprinting was used Inkredible+ device. Optimization of experimental parameters for creating a scaffold that can be used in tissue engineering is a demanding process. As it is the first stage of developing the blood vessel, there is a high importance of ensuring they are formed well and with the desired properties. This innovative approach may be promising for further fabrication of a blood vessel.

Electrospinning is highly used technique in the tissue engineering field, particularly in biomedical application [1]. The constricted concepts of this process are based on generate nonwoven nanofibers. The method involves high voltage electricity which is applied to the liquid solution and a collector, which lets the solution force out from a nozzle forming a jet. The jet formed fibers under influence of electrostatic forces concentrated and deposited these on the collector. Main objective of this study was to fabricate gelatin scaffolds with micro/nano-scale for successful wound dressing. Gelatin can mimic the chemical composition, physical structure and structure of the native skin extracellular matrix (ECM). However, the first and main principle in this study is the optimization of parameters of the electrospinning process. The used parameters have a crucial role in obtaining suitable fibers for further cell seeding and cell growth in vitro. With the use of series of various biocompatible polymers and solvents, solutions were tested in various electrospinning settings in order to produce microscale fibers. The scaffolds were analysed with scanning electron microscope images for fiber diameter measurement.