A trial was conducted to evaluate the effect of live yeast culture Saccharomyces cerevisiae (SC) on the glutathione content of liver and gastric mucosa.  Twenty  male New Zealand white rabbits, aged 5 weeks, were studied in 2 groups. Groups were control (basal diet) and  SC (added 3 g/kg diet). The experiment lasted for 90 days. Blood samples were obtained by ear venipuncture on the 90th day. According to results, glutathione concentration of both liver and gastric mucosa were increased by the addition of SC (p<0.05). S.cerevisiae may be used for antioxidant supplement due to the maintain of glutathione metabolism. SC intake may contribute to enhanced quality of life by increasing antioxidant protection. More studies would be necessary to elucidate the effects of yeast culture supplementation on concentration of glutathione in animals and humans.

Glutathione is the important antioxidant agent that is used for body detoxification system. Because of the fact that ıt is crucial for protecting health. A feeding trial was conducted to evaluate the effect of natural additives such as live yeast culture Saccharomyces cerevisiae (SC) and microalgae Spirulina platensis (SP) on the glutathione and leukocytes counts of rabbits. Forty male New Zealand white rabbits, aged 5-6 weeks, were studied in 4 groups. Treatments were control group, SC (added 3 g/kg diet), SP (added 5% of the diet) and, SC and SP (added 3 g/kg diet and added 5% of the diet) respectively. The experiment lasted for 90 days and the blood samples were obtained by ear venipuncture on the 90th day.In conclusion, according to the results of this study, although not statistically significant, supplementing rabbit with S. cerevisiae or S. platensis had increased on glutathione values. Glutathione tend to be positively correlated with the addition of SC or SP. No significant difference in white blood cell counts was evidenced, even if lymphocyte counts tended to increase and neutrophil counts to decrease in rabbits fed SC or SC+SP. The determination of biological consequences (antioxidant potential, resistance to diseases, and improvement of nutritional status) requires further investigations.

ÖzAmAÇ: Oksidatif stres belirteçleri diyabetes mellitus (DM) ve komplikasyonları ile ilişkilendirilmiştir. EBC (Exhaled breath condansate), solunum havasının soğutularak yoğunlaştırılması temeline dayanan bir yöntemdir. Çalışmada mikroalbüminürisi olan ve olmayan DM olgularında ekshale soluk havasındaki oksidatif belirteçlerinin (Nitrik oksit (NO), 8-isoprostan, lökotrien E4 (LTE4)) mikroalbüminüri ve solunum fonksiyon testleri ile ilişkisinin araştırılması amaçlanmıştır. GErEÇ ve YÖNtEmlEr:Çalışmaya Tip 2 DM tanılı 47 hasta alındı. Grup 1; mikroalbüminürisi olmayan 25 (K/E:17/8) ve Grup 2; mikroalbüminürisi olan 22 (K/E:14/8) hastadan oluşuyordu. Yirmibeş sağlıklı gönüllü (K/E:18/7) çalışmaya eklendi. Hasta ve kontrol gruplarına solunum fonksiyon testleri ve 24 saatlik idrarda mikroalbümin ölçümü yapıldı. Yoğunlaştırılmış soluk havası örnekleri alınarak kolorimetrik yöntemle NO ve EIA yöntemiyle 8-İsoprostan ve LTE4 düzeyleri çalışıldı.BUlGUlAr: Her iki hasta grubu ve sağlıklı kontrollerde oksidatif belirteç düzeyleri arasında istatistiksel olarak anlamlı fark yoktu. Hastaların mikroalbüminüri düzeyleri ile oksidatif belirteç düzeyleri arasında da korelasyon saptanmadı. Grup 2'deki hastaların solunum fonksiyonlarında anlamlı düşüklükler saptandı. SONUÇ:Çalışmamızda, EBC yöntemiyle, mikroalbüminürisi olan ve olmayan diyabetik hastalar arasında oksidatif stres markerleri bakımından anlamlı bir fark bulunmamıştır. Bu durum olgu sayısının sınırlı olmasına veya soluk havasını etkileyen dış faktörlere bağlı olabilir. Diyabetik hastalarda EBC yöntemiyle yapılacak yeni ve kapsamlı çalışmalar oksidatif stres ve nefropati ilişkisi açısından anlamlı sonuçlar ortaya koyabilir. ANAhtAr SÖzcüKlEr: Oksidatif stres, Diyabetes mellitus, Mikroalbüminüri, EBC ABStrActOBJEctIvE: Oxidative stress has been found to be associated with diabetes mellitus (DM) and its complications. Exhaled breath condansate (EBC) is a method based on air cooling and condensation. This study aims to investigate a possible association between oxidative markers (8-isoprostane, Nitric Oxide (NO), Leukotriene E4 (LTE4)) in exhaled breath and microalbuminuria in diabetic patients and determine the effects of diabetes on respiratory functions. mAtErIAl and mEthODS: Forty-seven patients with type 2 DM were enrolled. Group 1 consisted of 25 patients without microalbuminuria (F/M:17/8) and Group 2 of 22 with microalbuminuria (F/M:14/8). Twenty-five healthy volunteers (F/M:18/7) were also included. Respiratory function tests and 24h urine microalbumin measurements were performed in all groups. NO was measured with colorimetry, 8-isoprostane and LTE4 levels were determined with EIA in EBC samples. rESUltS:Oxidative marker levels were similar among the patient groups and healthy controls. No correlation was observed between microalbuminuria and oxidative markers. Respiratory functions were significantly lower in Group 2.cONclUSION: There was no significant difference in oxidative markers measured with EBC for diabetic patients with or without microalbuminuria. We a...

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