CommentsLymphatic filariasis constitutes one of the major problem in tropical countries. This is a good study and the authors emphasized on the methods that used for diagnosis of lymphatic filariasis in Cx. quinquefasciatus mosquito, which is helpful to the m a n a g e m e n t o f v e c t o r c o n t r o l programmes in the country. . PCR was carried out using DNA extracted from mosquito pools (15 body parts/pool) utilizing the primers specific for Wb-SspI repeat. PCR products were analyzed by hybridization ELISA using fluorescein-labeled wild type specific probes. The prevalence of infected/infective mosquitoes in PCR pools (3 pools/ site) was estimated using the PoolScreen TM algorithm and a novel probability-based method. Results: Of 45 batches of mosquitoes dissected, W. bancrofti infected mosquitoes were found in 19 and 13 batches, with an infection rate of 13.29% and 3.10% with mean larval density of 8.7 and 1.0 larvae per mosquito for two study periods in the Gampaha District. Total of 405 pools of head, thorax and abdomen were processed by PCR-ELISA for each year. Of these, 51 and 31 pools were positive for W. bancrofti in the two study periods respectively. The association of dissection based prevalence rates with PCR based rates as determined by the Pearson correlation coefficient were 0.176 and 0.890 respectively for the two periods. Conclusions: Data indicate that PCR-ELISA is more sensitive than the traditional dissection techniques for monitoring transmission intensity.