Objectives:To investigate the effect of methanolic extract of Ixora coccinea Linn. (MEIC) leaves against doxorubicin-induced cardiac toxicity in rats.Material and Methods:Albino Wistar rats were pretreated with the methanolic extract of Ixora coccinea Linn. leaves (200 and 400 mg/kg, orally) for 1 week followed with the simultaneous treatment with doxorubicin (cumulative dose of 15 mg/kg in six divided doses for 2 weeks) along with the extracts for the next 14 days. On the 22nd day hemodynamic parameters such as blood pressure and ECG were recorded. Biochemical study including biomarkers like creatine kinase – MB (CK – MB), lactate dehydrogenase (LDH), SGOT and SGPT, tissue antioxidant markers viz. catalase (CAT), superoxide dismutase (SOD) and extent of lipid peroxidation viz. malondialdehyde (MDA) was estimated. Histopathology of heart was also done to assess the cardioprotective effect.Results:Pretreatment with MEIC significantly reduced (P<0.01) the ST segment elevation and also maintained the BP (P<0.01) close to normal. The MEIC significantly reduced the elevated level of biomarkers like CK - MB, LDH, SGOT, SGPT (P<0.01) near to normal, the MEIC also increased the tissue antioxidant markers viz. CAT, SOD and decreased the level of MDA (P<0.01) in cardiac tissue by dose-dependant manner. The histopathology of heart also further confirmed the cardioprotection provided by the methanolic extract of Ixora coccinea Linn. leaves.Conclusion:The results suggest a cardioprotective effect of Ixora coccinea Linn. leaves due to its antioxidant properties.

The aim of study was to evaluate cardio protective activity of fulvic acid in Isoprenaline induced cardiac toxicity. In current investigation, female/male wistar rats were divided into five groups, normal, control, Fulvic acid 100mg/kg, Fulvic acid 200mg/kg, and Fulvic acid 300 mg/kg. Isoprenaline 85 mg/kg was administered on 29 th and 30 th day during study period to all except normal group. Fulvic acid was administered to respective groups once daily for total 30 days, on the last day of study, the animals were anesthetized to record ECG and BP (by cannulating carotid artery), blood was collected from carotid artery and SGOT, LDH And CK-MB were estimated, Animals were sacrificed to isolate heart and preparation of tissue homogenate. The antioxidant status is analyzed by measuring MDA content, SOD CAT, GSH activity. The tissue sample is also preserved for histological studies. Isoprenaline causes cardiac damage which was manifested by alteration in serum cardiac markers, antioxidant markers, ECG and hemodynamic and histological changes. These alterations were restored due to treatment with fulvic acid 300mg/kg. With data obtained in study it have been concluded that fulvic acid treatment for 4 weeks protect the heart of rat from cardiotoxicity as a result of isoprenaline administration.

In the present carrageenan induced chronic pain model we have determined the role of analgesics in the reversal and inhibition of the state of chronic hyperalgesia. While considering the characterization of the present model our observations suggest the importance of a spinal COX-2 mechanism, a spinal action of systemically delivered drugs in the face of peripheral inflammation.

Background: This investigation was aimed to explore the anxiolytic potential of Phyllanthus amarus standardized extracts and predict probable role of marker phyto constitutents. Objective and Methods: Three standardized extracts of Phyllanthus amarus plant viz. standardized aqueous extract of Phyllanthus amarus whole plant (PAAE), standardized methanolic extract of P. amarus leaf (PAME) and the standardized hydro-methanolic extract of P. amarus leaf (PAHME) were tested in the classical animal models of anxiety vise: Elevated plus-maze model and Light & Dark Exploration test. Results: The lower doses of the tannin rich extract (PAHME) of the P. amarus possess significant anxiolytic activity compared to lignin rich (PAME) and aqueous extracts (PAAE), while at a higher dose (400mg/kg) the results of all three extracts appears to be potentially sedative. While the molecular docking studies support these probable anxiolytic and sedative effects of the Phyllanthus amarus extracts could be due to the interaction of tannins and lignans with the GABA-benzodiazepine receptor complex. Conclusion: The results of the present study indicate that the tannin-rich extract of the P. amarus may have potential clinical applications in the management of anxiety. It can be further studied for optimum dosage to be used as a future of anti-anxiety drug development or as a standardized Phytomedicine.

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