Purpose To evaluate the effect of different 3D printing orientations on internal and seating accuracy of implant surgical templates fabricated by a digital light processing (DLP) printer. Materials and methods A single maxillary model with a missing central incisor was used to design a surgical template for single implant placement. According to the printing orientation, three surgical template groups were included in the study: horizontal (H), angled (A) and vertical (V) (n = 10). For the H group, the templates were produced parallel to the printing platform, while for the V group, the templates were perpendicular to the platform. The A group templates had a 45° angle orientation to the platform. Each template was scanned at the fitting surface and after seating on the master model. The internal accuracy involved measuring the trueness and precision of the internal surface, while for the seating accuracy, the vertical discrepancy after seating the template was measured. To determine the difference among the groups, ANOVA test was applied followed by Tukey post hoc tests (α = 0.05). Results The H group had the lowest internal surface inaccuracy (trueness = 100.7 μm; precision = 69.1 μm) followed by A (trueness = 114.0 μm; precision = 77.3 μm) and V (trueness = 120.3 μm; precision = 82.4 μm) groups, respectively (p < 0.001). Similarly, the H group had the most superior seating accuracy (543.8 μm) followed by A group (1006.0 μm) and V group (1278.0 μm), respectively (p < 0.001). Conclusions The orientation of 3D printing of implant surgical templates fabricated by the DLP desktop printer influenced the accuracy of the templates. The horizontally printed templates consistently exhibited superior accuracy. To reduce deviation of implant placement, it is recommended to print the surgical templates with their largest dimension parallel to the printing platform.

The fabrication of durable and low-cost nanostructured materials remains important in chemical, biologic and medicinal applications. Particularly, iron-based nanomaterials are of central importance due to the ‘noble’ features of iron such as its high abundance, low cost and non-toxicity. Herein we report a simple sol–gel method for the synthesis of novel iron–titanium nanocomposite-based material (Fe9TiO15@TiO2). In order to prepare this material, we made a polymeric gel using ferrocene, titanium isopropoxide and THF precursors. The calcination of this gel in air at 500 °C produced Fe-Ti bimetallic nanoparticles-based composite and nano-TiO2 as support. Noteworthy, our methodology provides an excellent control over composition, size and shape of the resulting nanoparticles. The resulted Fe-based material provides a sustainable catalyst for selective synthesis of anilines, which are key intermediates for the synthesis of several chemicals, dyes and materials, via reduction of structurally diverse and functionalized nitroarenes.

Cholestasis is characterized by impaired bile flow which results in inflammation, cirrhosis, and ultimately liver failure. The current study is aimed to evaluate the anti-cholestatic effect of silymarin against α-naphthylisothiocyanate (ANIT) induced cholestasis. Mice were gavaged with various doses of silymarin or ursodeoxycholic acid (UDCA) for 19 days. Then they were challenged with α-naphthylisothiocyanate (ANIT) and after 48 hours the animals were sacrificed to obtain blood and liver sections. Serum levels of bilirubin, aspartate transaminase (AST), alanine transaminase (ALP), and liver histology were analyzed. mRNA expression of selected transporters (Bile salt export pump (BSEP) and sodium taurocholate cotransporting polypeptide (NTCP)) and proteins (farnesoid x receptor (FXR) and Cytochrome P450 Family 7 Subfamily A Member 1 (Cyp7a1)) involved in bile acids biosynthesis, excretion and uptake were also evaluated by quantitative PCR. The results indicated that the serum levels of bilirubin, AST, and ALP were significantly higher in a cholestatic model group as compared to an untreated control group. However, in silymarin groups, the serum level of these parameters is significantly lower than in a cholestatic model group. Liver histology also showed that silymarin prevents ANIT-induced hepatic injury. mRNA expression of FXR, BSEP, and NTCP was downregulated and expression of Cyp7a1 was upregulated in a cholestatic model group as compared to an untreated control group. However, in silymarin treatment groups, the expression of FXR, BSEP and NTCP was upregulated and the expression of Cyp7a1 was downregulated as compared to the cholestatic model group. In conclusion, silymarin could alleviate hepatic injury by modulating the expression of genes involved in bile acid homeostasis.