Microbiome studies are attracting increasing interest, especially in human health applications, where their use for disease prognostics, diagnostics and treatment can have immense effects on life quality. The settings in the microbiome data preprocessing stage can lead to the great variability of the generated operational taxonomic unit (OTU) tables, reflected in the size and sparseness of this data matrix. As there are still no solid guidelines on the best practices, it is valuable to assess which machine learning algorithms provide higher stability of results under variable preprocessing settings. In this study, we have generated OTU tables using data from the Moving pictures of human microbiome study using two different reference databases (Greengenes and Silva) and four levels of the similarity threshold (ranging from 90 to 99%), processed in the QIIME bioinformatics package. The results of the two best-performing classification and clustering algorithms are presented in detail: Random Forest classifier (RF) and Spectral clustering (SC). The random forest classifier has outperformed spectral clustering in terms of accuracy. As the rate of data generation increases, while the cost of labeling remains high, further improvement of clustering performance and ensemble approaches should be explored.

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Ovaj rad daje prikaz sistema za detekciju stresa kod pilića tovljenika na osnovu analize zvuka njihovog oglašavanja. Skup obeležja na osnovu kojih ovaj sistem vrši prepoznavanje čine: energija, snaga, kvadratna sredina, džiter, šimer, prosečna visina zvuka, odnos harmonik-šum, izlazi iz mel-filtar banke i mel-frekvencijski kepstralni koeficijenti. Kao klasifikator iskorišćen je klasifikator na bazi vektora nosača. Tačnost sistema na nivou frejma od 50 ms varira od 61 do 88%, u zavisnosti od starosti tovljenika i izbora obeležja.

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