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Microfluidic technology is a science and technology that can accurately manipulate fluids in micro-sized channels. In recent years, microfluidic devices have attracted wide attention due to its easy manipulation, miniaturized size, high throughput and precise control, which provide a potential platform for antibody screening. This review paper provides an overview of recent advances in microfluidic methods application in the field of antibody preparation. While hybridoma technology and four antibody engineering techniques including phage display, single B cell antibody screening, antibody expression and cell-free protein synthesis are mainly introduced, important advances of experimental models and results are also discussed. Furthermore, the authors expound on the limitations of current microfluidic screening system and present future directions of antibody screening platform based on microfluidics. Antibody preparation on microfluidics combined with other technologies has huge application potential in the field of biomedicine, and it is anticipated to be further developed.

Cell size is an important parameter for cell analysis and manipulation. Cell sample with similar size distribution can improve the performance in cell screening, cell electroporation and electrofusion. One approach for cell purification is to utilize constriction structures such as dams to trap cells in microfluidics. This paper presents a centrifugation and negative-dielectrophoresis assisted size-based cell separation method. The goal is to separate cell sample and get purified cells by size-based screening. We designed and fabricated a microfluidic chip with five octagon-shape screening-dams within a flow-through channel network. The radius of the octagon was from 10mm to 26mm, and the array space was 4 mm. The octagon-shape screening-dam contained hundreds of micro-filter and micro-dam structures. Each micro-filter was composed by two micro-dam structures. The width of filter between two adjacent micro-dams is 40, 30, 20, 15, 10 mm, respectively. After loaded cell sample into the central reservoir, the microfluidic chip was revolved in high speed. Cells with big size were immobilized and captured into micro-filter under centrifugal force. To avoid too much cells are concentrated into micro-filters and blocked these structures, negative-dielectrophoresis was applied per 2 minutes, to push cell leave to filter and move into independent chamber between two adjacent octagon-shape screening-dam arrays. In the end, purified cells with similar size were separated and collected into each independent chambers.