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The Hedonic and Eudaimonic Motives for Activities-Revised scale (HEMA-R) is one of the most extensively used instruments to assess how people pursue well-being. The main aims of the present research were to translate HEMA-R into Chinese and test its construct and predictive validity as well as measurement invariance across gender. In Study 1, we conducted confirmatory factor analysis with data containing 1090 Chinese undergraduates, and replicated the two-factor model which has been found in other studies. Furthermore, the measurement invariance across gender was supported throughout the multi-group confirmatory factor analysis. Study 2 replicated these results and further found the HEMA-R had satisfactory predictive validity in measures of well-being, social support and smartphone addiction. All the findings indicate that the HEMA-R is reliable and valid to measure hedonic and eudaimonic motives, and it could be applied generally across gender in Chinese adults.