The purpose of this article is to synthesize some novel 1,8-Naphthyridine-3-Carboxylic acid derivatives, analyze them, and assess their antibacterial potential. With the help of elemental analysis, IR, NMR, and mass spectrum data, the synthesized derivatives were identified. Derivatives' antibacterial activity was determined using the cup and plate diffusion method. At doses of 50 μg/ml and 100 μg/ml, the substance demonstrated substantial antibacterial potential against the tested strains. To predict the pharmacokinetic properties (ADME) of these derivatives, in silico investigations were also carried out. For the current study, the in silico Swiss ADME assisted results were shown to be suitable for the derivation and synthesis of efficient antibacterial drugs.