Multinucleate cells are widespread in nature, yet the mechanism by which cells fuse their plasma membranes is poorly understood. To identify animal fusogens, we performed new screens for mutations that abolish cell fusion within tissues of C. elegans throughout development. We identified the gene eff-1, which is expressed as cells acquire fusion competence and encodes a novel integral membrane protein. EFF-1 sequence motifs suggest physicochemical actions that could cause adjacent bilayers to fuse. Mutations in the extracellular domain of EFF-1 completely block epithelial cell membrane fusion without affecting other perfusion events such as cell generation, patterning, differentiation, and adhesion. Thus, EFF-1 is a key component in the mechanism of cell fusion, a process essential to normal animal development.

Currently there is no adequate experimental model available whereby the lethal infiltration of malignant lymphoma to the eye and CNS can be studied. Variant S49 mouse lymphoma cells that exhibit cell-cell adhesion properties (named Rev-2-T-6) were inoculated intraperitoneally into Balb/C mice at the ages of 6-60 days postnatal. Mice inoculated between days 6-11 postnatal developed signs of eye and CNS involvement with an apparent peak (58% of mice) at day 7. None of the mice inoculated beyond day 11 exhibited such signs. Histological analysis of these sites revealed tumorous infiltrates into a variety of structures in the orbit, intraocular tissues, along the optic nerve and in the brain. Additional analysis of the histopathological data, based on the structures demonstrating the highest frequency of lymphoma infiltration, suggests preferred routes of lymphoma entry to the brain and eye. Thus, entry to the brain can occur mainly through the choroid plexus and cranial nerves or cranial nerve ganglia. Entry to the eye may occur from the brain (along the optic nerve), and through hematogenous infiltration of orbital structures. No data were found that would support retrograde infiltration of the lymphoma from the eye to the brain. These findings present an experimental model for addressing the molecular mechanisms that govern homing of malignant lymphoma to the eye and brain, as well as the development of experimental therapeutic modalities for malignant lymphoma in these organs.

A doença inflamatória intestinal (DII) é uma enteropatia crônica idiopática que provoca distúrbios gastrointestinais em cães e gatos. Ocorre em animais com meia idade e as manifestações clínicas mais comuns incluem êmese e diarreia intermitente. A análise histológica permite classificar a DII de acordo com o infiltrado celular e pode ser obtida principalmente por laparotomia ou endoscopia. A terapia é baseada na dieta, oferta de fibras e medicações imunossupressoras. O objetivo do estudo é relatar três casos de DII, confirmada após biopsia. Neste relato os animais foram submetidos em procedimento de laparotomia e endoscopia digestiva alta para coleta de amostra histopatológica. Todos os animais foram diagnosticados com DII, contudo houve complicações pós-operatórias. O manejo dietético com proteínas de alta digestibilidade, suplementação de fibras e prednisona, azatioprina ou ciclosporina se demonstraram eficazes nestes pacientes.

The protected Mediterranean woodland habitats in Israel are undergoing tree encroachment, resulting in loss of open patches with herbaceous vegetation. We suggested that this process results in a ground spider community dominated by shade-loving species. At three Mediterranean woodland sites located along a rainfall gradient, we examined the effects on the ground-spider community of experimental removal of the woody vegetation in 1000 m2 plots by cutting and overall plant biomass reduction by grazing and browsing by livestock. Pitfall traps were placed in replicated plots of four treatments (control, cutting, grazing/browsing, and cutting together with grazing/browsing) and in two different habitat patch types (open, woody). ANOVA and multivariate analyses were performed on family abundance by treatment and habitat patch type. Tree-cutting reduced the number of families in plots at two of the three sites. Grazing did not have a significant effect on the number of families or on the ground spider community composition. The spider community of cut-woody patches was more similar to that of open patches than to that of uncut woody patches. Most spider families separated along an axis of open versus woody patches, with woody habitat families predominating at all sites. Families typical of open habitats were positively associated with cut-woody patches as well. The overall effect on ground spider diversity of such manipulations may depend on the scale of habitat changes

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