To estimate the antimicrobial effect of 0.2% Chlorhexidine and HiOra mouth wash in reducing the bacterial load. Objectives:1.To evaluate the clinical efficiency of 0.2% chlorhexidine as a pre rinsing mouthwash. 2.To evaluate the clinical efficiency of HiOra mouthwash as a pre rinsing mouthwash. 3.To evaluate the microbial load without use of pre-rinsing mouthwashes. Materials and Methods:This study is a randomized clinical study in which 45 patients of both sexes within the age range of 20 to 70 from both the genders were selected. Patients were categorized into three groups (group A, B, C) based on random selection.Un-stimulated saliva was collected from all the three groups prior to the procedure. Group A was given normal saline mouth rinse, group B was given 0.2% Chlorhexidine, and Group C was given herbal mouthwash (HiOra) as a pre procedural rinse. After rinsing with mouthwashes saliva samples are further collected. All the samples collected were sent to the laboratory for analyzing colony forming units (CFU).Conclusions: Pre-procedural rinse significantly reduce the amount of microbial load of aerosols. chlorhexidine was found to be more effective than herbal mouthwash.