This study aimed to analyze epidemiological aspects and spatial characteristics of Lesptospira spp. infection among sheep in Pernambuco state, Brazil. A total of 426 samples were collected from unvaccinated animals aged 1 year or older, and submitted to Microscopic Agglutination Test (MAT). To study the risk factors, a standardized investigative survey of sheep farmers, with objective questions, was conducted to establish the general characteristics of the farm property production, reproductive management, and health plan. From 426 samples analyzed, 83 (19.5%; CI 15.9 - 23.6%) were positive; among them, the most prevalent serovar was Autumnalis (32.4%). Significant differences were observed between gender (OR = 0.38; CI 0.14 - 0.99), production system (OR = 2.03; CI 1.07 - 3.84), types of herd (OR = 2.28; CI 1.39 - 3.72), absence of rodents to the grain storage (OR = 0.55; CI 0.34 - 0.91), and restriction to surface waters (OR = 0.60; CI 0.37 - 0.99). Leptospira spp. infection is disseminated in sheep herds, and multiple factors can influence its occurrence. Health education programs and worker training in prevention and control of leptospirosis and other infectious diseases may be useful to reduce infection rates and economic losses caused by this disease.

Bacteria of the genus Brucella are widespread in many countries. These microorganisms can infect humans and many wild and domestic animal species. These bacteria have zoonotic potential, and can cause economic and public health problems since they can be transmitted by direct contact with sick animals, through consumption of contaminated milk, raw meat and its derivatives (Soares et al., 2015). Brucellosis is considered a chronic evolving disease, unusual in horses, predominantly caused by Brucella abortus. However, it is not characterized by reproductive disorders in horses, but primarily by abscess in the cervical region, bursa, tendons, and joints. Transmission is likely to occur via ingestion of contaminated water and pastures, especially in areas endemic for bovine brucellosis (Ribeiro et al., 2008). The slaughterhouse is a strategic point for obtaining information about the animal and animal products, edible or not. This study investigated the presence of anti-Brucella spp. immunoglobulins in the serum samples from horses slaughtered in a slaughterhouse in southern Brazil, to estimate the frequency of Brucella spp. antibodies and determine the spatial distribution of the cases.Keywords: equine, abattoirs, brucellosis, equine, fluorescence polarization, complement fixation RESUMO Objetivou-se investigar a presença de imunoglobulinas anti-Brucella

The objective of this study was to investigate the presence of anti-Leptospira spp. antibodies in serum samples of horses slaughtered in an abattoir, under the Brazilian federal food inspection service, in the southern region of Brazil. We tested 767 blood samples from adult horses slaughtered from April to May, 2013. The animals came from 45 municipalities in the states of Rio Grande do Sul, Santa Catarina, and Paraná. For the diagnosis we used the microscopic agglutination test (MAT). The results showed that 687 horses reacted to at least one of the 24 serovars of Leptospira spp., with titer equal to or greater than 100, representing 89.57% (95% CI: 87.41%-91.73%). The most likely serovars were Patoc (9.91%), Butembo (9.13%), Australis (7.82%), and Bratislava (5.87%). There was no significant difference (p = 0.2795) in the number of positive animals by state. The proportion of MAT-positive males and females differed significantly (p = 5.4444 x 10 -5 ) since 85.26% (95% CI: 82.82%-88.70%) of the males and 94.44 (95% CI: 92.07%-96.81%) of the females were reactive. The results of this study demonstrate a high rate exposure to several serovars of Leptospira in slaughtered horses. For the protection of animal, public, and occupational health, we suggest attention to infections in this host in order to reduce the risk of leptospirosis. Key words: Horses. Immunoglobulins. Leptospirosis. Microscopic agglutination. Slaughterhouse. ResumoObjetivou-se investigar a presença de imunoglobulinas anti-Leptospira spp. em amostras de soros sanguíneos de equídeos abatidos em matadouro-frigorífico, sob Serviço de Ins peção Federal, localizado na região Sul do Brasil. Utilizaram-se 767 amostras de sangue de equídeos adultos, abatidos no período de abril a maio de 2013. Os animais foram provenientes de 45 municípios dos Estados do Rio Grande do Sul, Santa Catarina e Paraná. Para o diagnóstico foi utilizado o teste de soroaglutinação microscópica (SAM). Foram observados 687 equídeos sororreagentes a pelo menos uma das 24 sorovariedades utilizadas de Leptospira spp. com título igual ou superior a 100, representando 89,57% (IC 95%: 87,41% -91,73%). As sorovariedades mais prováveis foram Patoc (9,91%), Butembo (9,13%), Australis (7,82%), Bratislava (5,87%). Não houve diferença significativa (p = 0,2795) entre as proporções de reagentes a

RESUMOA Vigilância em Saúde, componente estruturante do SUS, está alicerçada sobre 4 pilares: Vigilância Epidemiológica, Sanitária, Ambiental e Saúde do Trabalhador. É na Saúde do Trabalhador que se insere a Comissão Interna de Prevenção de Acidentes (CIPA) e o médico veterinário, profissional habilitado para compor e atuar em tais grupos de trabalho. Objetivou-se realizar o diagnóstico de situação do Hospital Veterinário "Governador Laudo Natel" (HV) da FCAV/ UNESP, Jaboticabal, sobre a saúde dos seus funcionários. Averiguando legalmente, segundo o Decreto-Lei n 5.452, a NR 5 e NR 7 que visam defender e monitorar a saúde do trabalhador. Participaram 33 funcionários do HV, que a partir de um inquérito sobre a Saúde do Trabalhador, identificou o grau de entendimento de cada trabalhador sobre seus direitos, conhecimentos específicos no exercício da profissão na área da saúde e a preocupação da instituição empregatícia com a saúde dos trabalhadores. Além do inquérito também foi realizado o levantamento sorológico dos funcionários para brucelose, doença crônica e de sintomas inespecíficos, pelo teste de triagem do Antígeno Acidificado Tamponado (AAT), este preconizado dentro do Programa Nacional de Controle e Erradicação de Brucelose e Tuberculose, Ministério da Agricultura, Pecuária e Abastecimento (PNCEBT/ MAPA), e também utilizado como triagem em Medicina Humana. Verificouse falhas na difusão e conscientização do trabalhador. Situação que pode aumentar a chance de acidentes, resultando na aquisição de enfermidades como a brucelose, além de outras. Pelo AAT não houve amostras positivas, não havendo a necessidade de realizar um teste confirmatório. Mesmo com a CIPA estabelecida na UNESP, observa-se que a mesma não está presente no HV. Existe a necessidade de implementar um grupo que tenha conhecimento da realidade do local, trabalhando junto aos funcionários para mitigar riscos e mostrar a importância dos próprios funcionários na prevenção de agravos a própria saúde. PALAVRAS-CHAVES: INQUÉRITO. BRUCELOSE. AAT. VIGILÂNCIA EM SAÚDE.AGRADECIMENTOS: Laboratório de exames sorológicos de leptospirose e brucelose.ÁREA TEMÁTICA: Saúde Pública.

Leptospirosis is widespread throughout the world. In the bovine species, economic losses are mainly associated with reproductive failures. Due to its importance, the objective of the work was to determine the serovarieties most related to bovine leptospirosis in southwest Goiás, Brazil. 2,006 samples were collected in two slaughterhouses in 14 of the 18 municipalities in that region. For the diagnosis, the blood serum samples were submitted to the Microscopic Soroagglutination test (SAM), against 32 serovarieties of leptospires, adopting a positive titer greater than 100. A geographical distribution of the positive animals and the main serovarities present in the southwest of Goiás was carried out, and also a questionnaire with the producers, containing epidemiological information, the vaccine being one of the prophylactic measures related to the infection, on the other hand it was also observed that vaccinated animals they have also been shown to be reactive. It was noted 1,406 (70.1%) cattle were seroreactive for at least one of the 32 serovarieties of Lepstospira ssp. tested. The most frequent serovarieties were Guaricura (30.80%), Wolffi (30.51%), Shermani (9.39%), Hardjo (7.54%), Bananal (4.27%) and Pomona (3.27) %). Serranópolis (77.9%), Perolândia (75.5%), Caiapônia (74.7%) and Jataí (73.2%) are the municipalities with the highest frequency of seroreagent animals. Through this research, a high index of seroreactive animals from the studied region was demonstrated, reinforcing the importance of leptospirosis infection in the Southwest region of the State, being necessary, therefore, the implementation of general measures of control and infection of the animals.