The effects of various Hevea brasiliensis (refined rubber seed oil) incorporated diets on wister albino rats were assayed. The results showed a general increase in total and free cholesterol levels of the rats within the duration of feeding. It was, however, observed that the increase in liver total cholesterol level was greater in groups E rats fed with (4% crude rubber seed oil incorporated diet) compared to groups C, B and D rats for the each of the corresponding weeks. It was also observed that the increase in liver free cholesterol level was greater in group E rats fed with (4% crude rubber seed oil incorporated diet) than groups A (control), B, C and D rats for each of the corresponding weeks.

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The effects of Hevea bransiliensis natural rubber on kidney free and total cholesterol concentration of experimental rats were analyzed. Forty albinos wister rats of either sex weighing 140-450g were used. The rats were divided into five equal groups marked A, B, C, D and E of eight rats each, and each group was fed on different dietary regime A, B, C, D and E respectively as indicated in tables 1 and 2. Diet A, (the control) had no rubber seed oil in the diet, whereas diets B, C, D and E (the experimental) had 2% refined rubber seed oil, 2% crude rubber seed oil, 4% refined rubber seed oil and 4% crude rubber seed oil by weight incorporated respectively. The results showed that the increase in mean kidney total cholesterol level was greater in group B (2% refined rubber seed oil incorporated diet) followed with groups A (control), C (2% crude rubber seed oil), E (4% crude rubber seed oil) and D (4% refined rubber seed oil) incorporated diets for the corresponding weeks. The results also showed an increase in kidney free cholesterol level throughout the period of experiment. It was observed that the increase in mean kidney free cholesterol level was greater in groups E and C (4% and 2% crude rubber seed oil incorporated diets) than group A control, B (2% refined seed oil incorporated diets) and D (4% refined rubber seed oil) for the corresponding weeks. Group D animals showed very little increase in mean kidney free cholesterol level as indicated in Table 4.