The Upper Cohansey River Watershed in southwestern New Jersey has a history of being affected by fecal coliform bacteria (FC). A study was undertaken to investigate the environmental factors associated with FC concentration. For 44% of samples taken throughout the watershed in 2012–2013, FC concentration exceeded the benchmark value. FC levels were related to air temperature, river discharge, and land use in stream buffers. Human sources of FC had been eliminated following research results published in 2009. Results of the study reported in this article suggest the need to further investigate wildlife sources of FC and to implement additional mitigation actions.