This study aimed to: evaluate the antibacterial efficacy of two natural irrigating solutions (Green Tea, and Propolis) with two different root canal sealers (AH Plus sealer, and Total Fill BC Sealer) against E. faecalis. Methods: One hundred single rooted teeth were selected. All root canals were prepared manually to #50 file. Finally the teeth were sterilized by gamma rays. Each root canal was inoculated with 0.5 ml of E.faecalis. Specimens were divided randomly into two main groups (50 specimens each) according to the irrigating solution; A1 (Green Tea), and A2 (Propolis). Each main group was divided into two subgroups with (25 specimens each) according to the type of sealer used B1 (AH Plus sealer), and B2 (TotalFill BC Sealer). The bacterial sampling counting process was done three times throughout the study. Five specimens from each subgroup were examined by SEM for presence or absence of E. faecalis. Results:The highest mean value of bacterial count was found in (Green tea) (6.50x10x10 ± 1.29x10x10) while the least mean value of bacterial count was found in (Propolis) (with less antibacterial effect 2x10x10 ± 1.33x10x10) with higher antibacterial effect. The highest mean value of bacterial count was found in (AH Plus Sealer) (5.40x10x10 ± 2.44x10x10) with less antibacterial effect while the least mean value of bacterial count was found in (TotalFill BC Sealer) (3.10x10x10 ± 1.54 x10x10) with higher antibacterial effect. Conclusions: Propolis is an effective intracanal irrigant in eradicating E.faecalis. On other hand TotalFill BC Sealer is more effective in reducing E. faecalis.