INTRODUCTIONUrinary tract infections (UTIs) refer microbial colonization of the urine and tissue invasion of any structure of the urinary tract (Aminu and Aliyu, 2015). Bacteria are most commonly responsible although yeast a viruses may also be involved 2014). Females are believed to be more affected than males except at the extremes of l because bacteria can reach the bladder more easily in women, partially due to the short and wider female urethra, and its proximity to the anus (Oleghe et al., 2014). Available scientific information indicates that bacteria easily travel up to the urethra from the rectum and thereby causing infection, of interest, is the fact that urinary tract infections in pregnancy increases during the gestational period, beginning from the sixth week of the first trimester and peaking in the 22nd to week of the second trimester (Turay 2014). At this period, approximately 90% of pregnant women develop ureteral dilatation which remains until delivery, leading to increase in bladder volume and decreased ABSTRACT Urinary Tract Infection (UTI) is an infection caused by the presence and replication of microorganisms in the urinary tract. UTI pathogens associated with pregnant women attending Federal Medical Center, Katsina. The research was a prospective study conducted among 126 antenatal attendees. Urine microscopy and culture technique were conducted for each participant. Statistical analysis of data was done using Graph Pad Prism Statistical software. Twenty participants had asymptomatic bacteriuria giving a prevalence of 19.8%. However age (P=0.66), educational status (P=0.19) and settlement (P=0.91) are not statistically significant. While tribe and gestational age (trimester) are found to be statistically significant (P = 0.02). Staphylococcus aureus is found to be the most prevalent organism (40%), followed by coagulase negative Staphylococcus (32%) and the least is Eschericia coli (4%). However, comparison between Hausa and Yoruba had shown that Hausa tribe has more chance of infected with Asymptomic bacteriuria during pregnancy and high risk thanYoruba tribe! Therefore, UTI has the potentiality to pose serious life threatening consequences when left untreated. This is more likely to be the case where access to or availability of timely and appropriate medical intervention due to inadequate numbers of health care providers.It is therefore, recommended that pregnant women should be subjected for urine laboratory screening for the detection of UTI during the first trimester and treatment follows immediately for the positive results.
Foods are not only intended to placate hunger and supply essential nutrients but also to prevent the nutrition-related diseases and to improve physiological and mental wellbeing of consumers. This led to the development of probiotic foods. Probiotics are said to be live microorganisms which when adequately (106/107 CFU/mL) administered confer health benefits to the host. Probiotic drinks both dairy and non-dairy have been found to be carriers of these organisms to their site of action. This research aims at determining the survivability of probiotic lactic acid bacteria in some locally produced drinks. The isolation of the organisms from yoghurt and “nono” were conducted using cultural methods and further characterized using biochemical tests. Cell surface hydrophobicity (CSH), cellular auto-aggregation assays (CAA), hemolytic activities, bile salt and NaCl tolerance test were conducted to determine the probiotic properties of the organisms. The survivability of the organisms was evaluated after standardizing the inoculum using 0.5 McFarland standard and then serial dilution, plating and counting of visible colonies. Results were analyzed by ANOVA using SPSS software version 20.0. Out of the ten isolated and biochemically characterized lactic acid bacteria, three Lactobacillus species showed probiotic status, with CSH values of (79%, 75.2% and 91.3%) and CAA index of (79.3%, 74% and 83.5%), respectively, however there is no statistically significant difference between the CAA and CSH values of the isolates (p = 0.13 and p = 0.5, respectively), which indicates that all the isolates had approximately equal probiotic capabilities. “Kunun zaki” showed the highest survivability rate with the probiotic status being maintained for three days, and counts ranging from 1.4 to 1.05 x 106 CFU/mL; whereas “zobo” showed the least duration as a probiotic (one day, and a count of 1.16 x 106 CFU/mL. There is a significant difference (p=0.02) in the growth of the organisms over the incubation period (5 days), however, there is no significant difference (p=0.82) between the growth of the organisms growing on different drinks, indicating that time after incubation is the critical determinant of probiotic status, and that the isolates can grow equally well in all the drinks tested. This research reported the isolation of Lactobacillus species confirmed to be probiotics from yoghurt and “nono”. Enumeration of probiotic LAB in all the drinks revealed the drinks were probiotic containing 106 CFU/mL for two (2) days with the exception of “Kunun zaki” that reached up to three (3) days as a probiotic drink. The pH level of all the drinks decreased and the drinks became acidic (pH range 6.0-1.8).
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