More release of mineral-P from organic P compounds (phytin and glycerophosphate, 200 mg L-1) was observed due to the action of 10 mg L-1 organic acids (formic, citric, lactic, malic).Lactic acid was found to be most effective followed by formic and citric acid. In general, malic acid showed poor response as compared to the other organic acids tested for P mineralization. The inorganic P (Pi) release from glycerolphosphate, by different organic acids, was 62-66% higher than that release from phytin at the concentration of 10 mg L-1. The maximum Pi release from glycerophosphate and phytin by the action of different organic acids varies between 7.50 mg L-1 to 8.26 mg L-1 and 4.64 mg L-1 to 5.10 mg L-1 respectively. The optimum time of incubation for more P mobilization from glycerophophate varies between 90 and 135 minutes while phytin required from 60 to 105 minutes. The efficiency of different organic acids towards mineralization of P follows the order: lactic acid > formic acid > citric acid > malic acid.

The phytase enzyme was collected from plants (cereal, legumes and oilseeds) and fungal sources and compared their efficiency to release inorganic P from organic P compounds. In general, fungi released more phytase after 21 days of growth and their extracellular activity was more than their intra-cellular counterpart. Plants are releasing 4 to 43% more phytase under P deficient condition as compared to the sufficient P condition. Oilseed crops releases higher phytase than legumes and cereal crops. But phytase released from legumes are discovered to be more efficient to hydrolyze organic phosphorus. Phytase release by Chaetomium globosum was exposed to be most efficient on as compared to the phytase produced by other tested fungi. Phytase originated from fungal sources are described to be more efficient than similar amount of phytase originated from plant sources.