One of the efforts to increase domestic production is improving superior soybean through plant breeding techniques. Improving superior soybean cannot be separated from the regeneration process. Regeneration via somatic embryo induction has the advantage of being bipolar. Types of explants, genotypes, media composition, and growth regulators play an important role in plant in vitro regeneration. This study aims to obtain a regeneration protocol for two varieties of soybeans, namely, Derap I and Devon I, through induction of somatic embryos. This research was conducted at Tissue Culture Laboratory of the Faculty of Agriculture, Andalas University, Padang, from July to November 2020. This study used a completely randomized design (CRD) that was carried out separately in one experiment with 4 levels of 2,4-D, namely, 10 ppm, 20 ppm, 30 ppm, and 40 ppm, which were repeated 5 times. The results showed that the concentration of 2,4-D 20 ppm was the best concentration for percentage of explants that produced somatic embryos in Derap I (35%), and Devon I (95%).

Gambir is a type of plantation plant that has many benefits. Gambir extract can be used for the food, cosmetic, pharmaceutical, and leather tanning industries. However, the availability of gambier has decreased in production. Hence, information and technology for propagating Gambir seeds in vitro is needed. The growth of plant explants can be impacted by the application of growth regulators and explant sources in-vitro. This study’s purpose was to ascertain the connection between BAP concentration and explant sources concerning the development of gambier shoots. In addition, it is also to obtain of BAP and the best source of explants for the induction of the gambier shoots. In this study, a Factorial Completely Randomized Design (CRD) with 6 treatments that were each repeated five times was used. There are two levels of explant source and three levels of BAP concentration, the first of which is the concentration of BAP (2 mg/l, 4 mg/l, and 6 mg/l) (the first node and the third node from the shoot). The findings demonstrated that, in general, 100% of shoots could form when BAP and explant sources were concentrated. The concentration of BAP and the origin of the explants significantly influenced the emergence time of the shoots, with the quickest time of budding occurring after 3.8 days at a concentration of 2 mg/l BAP at the third node. 6 mg/l BAP was the optimal treatment for increasing the number of gambier leaves and shoots.