The study investigated antibacterial potentials of Morinda persicifolia Buch. – Ham collected from Lang Sen Wetland Reserve, Long An Province in Vietnam. Antimicrobial activity of M. persicifolia Buch. – Ham was evaluated based on size of antibacterial zone of methanol leaf extract by agar well diffusion method, values of minimum inhibitory concentration (MIC) by micro-well dilution method, minimum bactericidal concentration (MBC) by spread plate method and MBC/MIC ratios of fruit decoction against five test bacteria. The results showed that the methanol leaf extract produced growth inhibition zones against test bacteria with diameters of smaller than 6 mm (2.41 mm -5.63 mm). The fruit decoction had MIC of 100 mg/mL for P. aeruginosa and 200 mg/mL for B. cereus, B. subtilis, S. aureus and E. coli; and MBC about 300 mg/mL to B. cereus and E. coli and 400 mg/mL to B. cereus, B. subtilis, S. aureus and P. aeruginosa. MBC/MIC ratios of the fruit decoction against test bacteria were from 1.5 to 4. Thus, the methanol leaf extract was considered antibacterial at weak level and the fruit decoction was considered bactericidal.

This study was conducted to develop an in vitro propagation procedure for Byblis liniflora Salisb. The process of seed sterilization and in vitro sowing achieved 95% efficiency. From the material aseptic stem segments, the shoot proliferation program was determined with the rate of 33.67 shoots/explants after 4 weeks of culture on nutrient medium (1/3 strength MS basal salts plus full Morel and Wetmore’s vitamins) with addition of a mixture of cytokinin (0.5 mg/L Kin and 2 mg/L BA) and then transferred to culture for another 4 weeks on nutrient medium. The rooting program from aseptic stem segments was to culture in nutrient medium supplemented with 0.5 mg/L NAA for 8 weeks. To produce a complete plant, single shoot separated from the proliferative clusters was transferred to a culture flask containing nutrient medium and cultured for 4 weeks. The in vitro shoot developed more branches and self-rooted to form a complete plant. The suitable growing substrate in the acclimatization and outdoor stages was a mixture of peat moss and perlite (1 : 2 w/w). The climatic conditions of Ho Chi Minh City and the Southeast of Vietnam were suitable for growing Byblis liniflora trees outdoors all year round.