Copper oxide nanoparticles were synthesized and characterized using by UV-Visible Spectroscopy, Scanning Electron Microscope, EDAX, X-Ray Diffraction and Fourier Transform Inferior Spectroscopy. Six feeds were prepared by using a fish meal, groundnut oil cake, wheat flour tapioca flour and different quantities of copper oxide nanoparticles such as 0,20, 40, 60, 80 and 100 mg/100g of feed. Feed utilization and haematological parameters were estimated after 21 days. The UV-visible absorption spectra show that CuO NPs were measured in wavelengths within 200 to 300 nm and exhibited strong adsorption at 220 nm. SEM images show that the Copper oxide nanoparticles were observed at the wavelength range of 9.18 nm. EDAX spectrum recorded two peaks located on the spectrum at 0.9 KeV and 8 KeV. XRD images were observed at the wavelength range from 75 nm. FT-IR spectrum was observed at the wavelength range from 400-4000 cm. The final condition factor of Tilapia was higher when compared to the initial. The feed consumption of Tilapia is higher in feed V (8.03) and lower in feed I (4.83). The feed conversion efficiency of Tilapia was higher in feed VI (7.6±6.9) and lower in feed IV (2.4+1.4). The feed conversion ratio of Tilapia was best in feed IV. Growth and the percentage growth of Tilapia were higher in feed III and I respectively. Assimilation of Tilapia was higher in feed IV and lower in feed V. Metabolism of Tilapia was higher in feed VI and lower in feed II. The WBC count is gradually increased from feed I to feed VI. Haemoglobin content decreased from feed I to feed VI. Haematocrit (Het) is higher in feed I (1.5%) and lower in feed VI (0.3 %). The platelets are increased with an increasing quantity of copper oxide nanoparticles in the feed.

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