OBJECTIVE: This study aims to evaluate the level of awareness, perception, specific knowledge about Ebola virus infection among men and women in Riyadh and Al-Kharj regions, KSA (kingdom of Saudi Arabia). METHODS: This cross-sectional study applied a 10-item anonymous, closed-ended, dichotomous questionnaire to collect information about knowledge regarding Ebola virus infection, modes of transmission, risk factors, symptoms, fatality rates and its risk to KSA among Saudis of various ages in Riyadh and Al-Kharj. RESULTS: Four hundred respondents were recruited for the study by random selection. Fifty eight percent of respondents did not have any knowledge about the mode of transmission, regions of the outbreak, seriousness of the infection, availability of treatment or vaccine or modes of prevention. Of the respondents, 164 (41%) reported having heard about EVI (Ebola virus infection), 271 (67.75%) considered EVI a serious risk during Hajj or Umrah and 239 (59.75%) responded that traveling to Africa is not safe. Social media was the main source of information among young adults while television and radio were more important sources among older participants. CONCLUSION: The findings suggest that the level of awareness about EVI in this study is low while the level of perception was just above average. An aggressive health education campaign is critical for increasing Ebola virus infection awareness.