The topic of Employee Ecological Behaviour (EEB) is gaining the attention of academics and practitioners, specifically on factors influencing it and the outcomes it leads. Despite an increasing number of recent studies on various green management practices and the competitive advantage they offer, the number of studies on EEB among academic staff is currently limited. Due to this, this paper aims to assess the effect of Green Culture (GC) on Green Satisfaction (GS) through the mediation of the ecological behaviour of academic staff in Malaysian public research universities. The Social Exchange Theory (SET) describes the association between GC, GS, and EEB. This cross-sectional study investigates how GC affects the GS of academic staff through EEB in Malaysian public research universities. A total of 299 valid responses were gathered and analysed using Smart PLS. The findings prove that green culture influences green satisfaction through the full mediation of EEB. The present study only focuses on academic staff in local public research universities. For future works, it is suggested to consider other factors that could facilitate the relationship between GC and GS. Policymakers can use the findings to understand the importance of green practices and develop a robust pro-environmental policy. It is believed that no prior study has attempted to use EEB as a mediator between GC and GS.