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A nano-revolution based on the green synthesis of nanomaterials could affect all areas of human life, and nanotechnology represents a propitious platform for various biomedical applications. During the synthesis of nanoparticles, various factors can control their physiognomies and clinical activities. Light is one of the major physical factors that can play an important role in tuning/refining the properties of nanoparticles. In this study, biocompatible monometallic (AgNPs and ZnONPs) and bimetallic Ag–ZnONPs (0.1/0.1 and 0.1/0.5) were synthesized under UV-C light irradiation from the leaf extract of Morus macroura, which possesses enriched TPC (4.238 ± 0.26 mg GAE/g DW) and TFC (1.073 ± 0.18 mg QE/g DW), as well as strong FRSA (82.39%). These green synthesized NPs were evaluated for their anti-diabetic, anti-glycation, and biocompatibility activities. Furthermore, their anti-cancerous activity against HepG2 cell lines was assessed in terms of cell viability, production of reactive oxygen/nitrogen species, mitochondrial membrane potential, and apoptotic caspase-3/7 expression and activity. Synthesized NPs were characterized by techniques including ultraviolet-visible spectroscopy, SEM, EDX, FTIR, and XRD. UV-C mediated monometallic and bimetallic NPs showed well-defined characteristic shapes with a more disperse particle distribution, definite crystalline structures, and reduced sizes as compared to their respective controls. In the case of clinical activities, the highest anti-diabetic activity (67.77 ± 3.29% against α-amylase and 35.83 ± 2.40% against α-glucosidase) and anti-glycation activity (37.68 ± 3.34% against pentosidine-like AGEs and 67.87 ± 2.99% against vesperlysine-like AGEs) was shown by UV-C mediated AgNPs. The highest biocompatibility (IC50 = 14.23 ± 1.68 µg/mL against brine shrimp and 2.48 ± 0.32% hemolysis of human red blood cells) was shown by UV-C mediated ZnONPs. In the case of anti-cancerous activities, the lowest viability (23.45 ± 1.40%) with enhanced ROS/NOS production led to a significant disruption of mitochondrial membrane potential and greater caspase-3/7 gene expression and activity by UV-C mediated bimetallic Ag–ZnONPs (0.1/0.5). The present work highlights the positive effects of UV-C light on physico-chemical physiognomies as well as the clinical activities of NPs.

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Wheat is a cereal that is majorly consumed around the world. There is a dire need for the advancement of wheat cultivars that are producing high yield. The experiment was conducted to characterize the spike related traits of wheat genotypes. Twenty wheat genotypes comprising three replications were experimented using randomized complete block design. The difference was significant among the studied twenty genotypes revealed by analysis of variance. Correlation Pearson’s revealed that the number of grains per spike (GPS) has positive relationship among all the studied traits such as the number of tillers per plant (NTP), spike weight (SWT), spike length (SL), and grain yield per plant (GYP). The GYP has a strong positive association with GPS (0.81**), GYS (0.78**), and SL (0.68**). The biological yield had a non-significant association with GYS (0.28) and GPS (0.13). All studied attributes have variations in the mean values which were represented by Radar analysis. The genotype Sadiq-21 was the best performing genotype among the SWT, GYS and BY followed by genotype Nawab-21 which performed best among the NTP, GYS and GPS and genotype Ghazi-19 which performed good among BY, SWT and GPS. The genotypes that were among the worst performing were Suren-10, Galaxy-13 and Borluag-16. The result of our study showed grain yield was mainly affected by NTP, GYS and GPS. For the improvement of wheat yield, these attributes must be improved for selection so that the yield of the wheat can be increased because these have a strong association with the grain yield.

The morphological traits of wheat grain strongly influence on yield and directly affect the grain yield of the crop. The necessity to overcome the human demand of wheat the grain yield should be intensified. The current experiment was organized to study the morphological traits of wheat and their association with yield. The experiment was done in the randomized complete block design including three replications and twenty genotypes under normal condition in cropping season of 2021. The result of analysis of variance indicates that there is highly significant difference among the studied genotypes, traits like spikelet per spike (SPS), flag leaf area(FLA), grain yield per spike(GYS), number of grains per spike(GPS), biological yield (BY) and grain yield per plant(GYP) showed the genetic potential and variability among them. While traits plant height (PH) and peduncle length (PL) are significant. Correlation analysis showed that grain yield per plant is highly associated with flag leaf area (0.78**) followed by biological yield which is highly correlated with plant height (0.77**) and number of grain per spike was also highly correlated with spikelet per spike (0.70**).While spikelet per spike and number of grains per spike correlated non-significantly with plant height (0.21ns), (0.23ns) and biological yield correalted non-significantly with spikelet per spike (0.21ns).The highest mean values recorded by the genotype G3 for the traits SPS (21.22), PL (16.74) and BY (23.33), followed by the genotype G14 for the traits SPS (23.22), PL (17.36), FLA (15), GPS (35.83) and by the genotype G19 for the traits PH (94.63), BY (22.23), GPS (33.16). Hence, in breeding or selection program, to develop the high yield of wheat cultivars in selection or breeding program, these yield related traits should have strong and positive association.