Iron (Fe) and Zinc (Zn) are essential nutrient elements for plant growth and development. Here, we observed the effects of Fe and Zn deficiency in seedlings of Areca catechu L. (areca palm), one of the most cultured palm trees in tropic regions. Results revealed that Fe deficiency causes strong chlorosis with the significantly decreased chlorophyll biosynthesis level and photosynthetic activities in the top third young leaf (L3) of seedlings. Zn deficiency caused light chlorosis in all three young leaves which slightly decreased chlorophyll biosynthesis and photosynthetic activities. Analysis of the Fe and Zn concentration in leaves and roots indicated that absorption and distribution of these two ions share cooperative pathways, since Zn deficiency caused Fe increasing, and vice versa. Therefore, we focused on the ZINC-IRON PERMEASE (ZIP) genes in areca trees. From the whole-genome data set we obtained, 6 ZIP genes were classified, and a phylogenetic tree was constructed with other 38 ZIP genes from model plants to find their potential functions. We also analyzed the expression pattern of AcZIP1-6 genes under Zn and Fe deficiency by transcriptomic approaches. With these results, we constructed an expression atlas of AcZIP1-6 genes in leaves and roots of areca seedlings with the dynamic expression levels under Fe and Zn deficient conditions. In conclusion, we provide evidence to understand the absorption and transport of nutrient elements, Fe and Zn, in the tropic agricultural plant A. catechu.

Diseases affecting the brain and spinal cord fall under the umbrella term “central nervous system disease”. Most medications used to treat or prevent chronic diseases of the central nervous system cannot cross the blood–brain barrier (BBB) and hence cannot reach their intended target. Exosomes facilitate cellular material movement and signal transmission. Exosomes can pass the blood–brain barrier because of their tiny size, high delivery efficiency, minimal immunogenicity, and good biocompatibility. They enter brain endothelial cells via normal endocytosis and reverse endocytosis. Exosome bioengineering may be a method to produce consistent and repeatable isolation for clinical usage. Because of their tiny size, stable composition, non-immunogenicity, non-toxicity, and capacity to carry a wide range of substances, exosomes are indispensable transporters for targeted drug administration. Bioengineering has the potential to improve these aspects of exosomes significantly. Future research into exosome vectors must focus on redesigning the membrane to produce vesicles with targeting abilities to increase exosome targeting. To better understand exosomes and their potential as therapeutic vectors for central nervous system diseases, this article explores their basic biological properties, engineering modifications, and promising applications.

Straw returning is an environmentally friendly method to improve soil health and agricultural productivity by reusing organic waste products. However, fields are often also treated with inorganic fertilizers, and the effect of the interaction between phosphate fertilizer application and straw return on crop yield remains unclear. Therefore, a full-factorial, two-year field experiment was conducted on sweet corn (NARC-16 in late 2021 and Kashmeri-19 in early 2022) to explore how crop yield may be optimized by combining straw return with efficient phosphate fertilization. The experiment involved the application of DAP, SSP, and NP (three different types of phosphates) and the application of crop waste byproducts, namely the residual stover left after sorghum and maize harvesting. We compared control fields with no crop waste or phosphate addition (CR0 and PS0) to experimental plots treated with various phosphates and straw return. Growth parameters such as days to emergence, tasseling, silking and maturity, emergence rate (emergence m−2), height of plant, number of leaves, leaf area per plant, and yield were evaluated, and the influence of the treatment on the economic value of crops was estimated. Phosphorus and straw return were applied at rates of 90 kg per hectare and 5 tons per hectare, respectively. The best P-crop straw combination treatment involved DAP (90 kg ha−1) with the incorporation of 5 tons’ ha−1 of maize straw, which resulted in delayed tasseling (50 days), early silking (68 days), taller plants (178 cm), improved thousand-grain weight (233 g), maximum biological yield (11,349 kg ha−1) and grain yield (3760 kg ha−1). The application of DAP with maize straw return resulted in the highest plant height, biological yield, and grain yield of sweet corn during the second year of the experiment, despite the first year’s yield being influenced by a natural disaster. This combined management strategy (using either DAP or crop residuals) was found to have a more a favorable cost–benefit ratio (BCR) efficiency. In conclusion, increasing the use of crop residuals can help reduce the expensive application of synthetic mineral fertilizers like SSP and NP, while significantly increasing sweet corn production and improving profit margins. Considering the importance of environmental friendliness and sustainable agriculture, the combined use of DAP and straw return is considered a viable method.