Article InfoDiabetes mellitus (DM) is one of more inveterate disease with wasteful complication, and the middle east is likely to onus one of the greatest problems of diabetes worldwide in the coming year. To measure the effectiveness of an educational program on adolescents knowledge about insulin shot technique. A research control trial was carried out at AL-fahiaa specialist to center in AL-fahiaa general hospital from 1 st November, 2015 until 21 th July, 2016. Non-probability sampling technique (purposive sample) base to criteria (diabetes mellitus type 1, used insulin injection and ability to read and write).The educational program was designed to improve patient knowledge toward insulin shot technique. This program was implemented through four steps: pre -program, program intervention, post test program and follow up. The educational activities employed several teaching and learning strategies. A questionnaire was designed as a tool for data collection for the purpose of the study. It was composed of three major parts, the first one concerning demographic data, medical history and third was divided into general information about DM (15) item and insulin shot technique administration questions (14) item. Data were collected and analyzed through the application of the descriptive statistical means (Frequency and percentage) and inferential statistical data analysis approach (one -way analysis of variance ANOVA test). Patients who had participated in the study had benefited from the implementation of educational insulin shot technique program and change had occurred to their knowledge about insulin administration technique (a mean scores of pre-test were improved in post-test Ι and post-test ΙΙ). Insulin shot technique education program had had a positive effect on adolescents knowledge related to insulin shot technique. The program should include diabetes mellitus in school curriculum.