Background: An assessment of the follicular reserve is essential to define the follicular status of the ovary. Various methods have been utilized to measure how many follicles are in the ovaries, including FSH, LH, AMH and AFC by Transvaginal ultrasound. This study aims to identify the correlation that exists between the different ovarian function markers. Objectives: To compare the relationship between serum AMH, FSH, and LH levels with AFC on day 3 of a menstrual cycle. Patients and methods: 100 infertile women in the department of obstetrics and gynecology at Al-Emameen Al-Khadimen Medical City were studied on day 3 of their menstrual cycle from April 1, 2014 to April 1, 2015. Measurements were taken for serum AMH, FSH, LH levels and AFC. Results: Median (range) serum levels of AMH, FSH, and LH were 2.45 ng/ml (0.24-6.5), 5.6 mIU/ml (2.1-19.3), 2.3 mIU/ml (1.1-10.0) respectively, and AFC was 15 (1-35). Serum AMH levels correlated more strongly with follicular count (r = 0.965 p ˂ 0.001) than levels of FSH (r =-0.446 p ˂ 0.001) and LH (r = 0.049 p = 0.625). Conclusion: Serum AMH levels showed a stronger correlation with the AFC on cycle day 3. This is an indication that AMH may demonstrate ovarian follicular status better than the hormone markers usually used.