The therapeutic value of the phenolic component and pure thymol was well known; this study comprised the extraction of crude phenol from two plants (Thymus vulgaris and Artemisia annua) which contain thymol with pure thymol and evaluate their effect on hematological and histological by using three different concentrations of each plant extract and pure thymol to tested them on lab mice. All the mice were allowed free access to water and feed for 21 days in laboratory conditions; orally, pure water was administered to the control mice (group I), while groups II, III, and IV were given orally with T. vulgaris, A. annua, combination of last two crude phenol plant extract 50:50 and pure thymol respectively. The levels of CHO, TRI, and HDL were significantly reduced in A. annua and T. vulgaris crude phenolic extract at 20 mg/ml as compared to the control treatment, while pure Thymol at 10 mg/ml gave the best result in reducing the levels of CHO, TRI, and HDL. In comparison to the control group, serum levels of liver enzymes in groups treated with in A. annua, T. vulgaris extract, and pure Thymol were substantially reduced in concentration: 20, 10 and 30 mg/ml, respectively.

The widespread use of broad-spectrum antibiotics has resulted in antibiotic resistance for many human bacterial infections, making multi-drug resistance a significant challenge in the treatment of infectious diseases. The antibacterial impact of phenolic plant extracts has been studied in new and different ways. The phenolic extract of Thymus vulgaris, Artemisia annua and pure thymol were used to study bacterial biofilm formation. In addition, the experiments were carried out to determine the lowest inhibitory concentrations in order to evaluate the antibacterial impact (MICs) against 20 isolations of Pseudomonas species. In this report, the plant extract was analyzed by HPLC techniques to determine the Thymol value. Although the Thymus vulgaris and Artemisia annua phenolic plant extracts and pure thymol concentrations (50, 25, 12.5, 6.25, 3.125, 1.250 and 0.006 mg/ml) (W/V)) were used through antibiotic and biofilm inhibition assay. The best results of MICs of T. vulgaris, A. annua extract and pure Thymol against bacteria isolates were 5.000 mg/ml, 1.250 mg/ml and 0.006 mg/ml, respectively. Stimulatory, the effect of T. vulgaris, A. annua extract and pure Thymol on biofilm formation were gave 10 mg/ml, 5mg/ml and 0.006 mg/ml respectively.

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