ABSTRAKAntioksidan diketahui dapat mencegah penyakit akibat kerusakan oksidatif oleh radikal bebas. Meskipun antioksidan sintetis tersedia secara komersial, tetapi tingkat toksisitasnya menjadi masalah. Oleh sebab itu penelitian untuk memperoleh sumber-sumber baru antioksidan yang potensial dari tumbuhan tetap diperlukan. Penelitian ini bertujuan mengetahui senyawa metabolit sekunder dan aktivitas antioksidan dari sejumlah tumbuhan hutan yang digunakan sebagai obat tradisional oleh Suku Dayak di Desa Petangis, Kabupaten Paser, Kalimantan Timur. Sampel tumbuhan yang digunakan antara lain kulit batang Guioa pterorhachis, akar Flagellaria indica, akar Nauclea officinalis, dan akar Clerodendrum adenophysum. Skrining awal fitokimia dari ekstrak etanol sampel menunjukkan adanya kandungan flavonoid, tanin, saponin, dan triterpenoid. Uji penghambatan radikal bebas menggunakan metode DPPH menunjukkan bahwa ekstrak etanol kulit batang Guioa pterorhachis memiliki nilai IC 50 lebih kecil dari 7,8 ppm dan akar Nauclea officinalis memiliki nilai IC 50 43,7 ppm. Aktivitas antioksidan kedua tumbuhan tersebut tergolong kuat sedangkan akar Flagellaria indica dan akar Clerodendrum adenophysum tidak aktif sebagai antioksidan karena menghasilkan nilai IC 50 lebih dari 200 ppm.
Bajakah and akar kuning are known as traditional medicine from peatlands, which are usually extracted from their natural habitats. Over extraction and illegal cutting have become a threat to the extinction of both species. Therefore, propagation and cultivation of those medicinal plants are necessary to be conducted outside forest areas to meet the demand of raw materials of traditional medicine and minimize the risk of species extinction. The experiment of stem cutting propagation of two bajakah species (Uncaria acida and Salacia sp.) and akar kuning (Fibraurea tinctoria) were conducted separately in two different nurseries. The results showed that stem cuttings of woody liana could produce more than one new shoot. The new shoots of U. acida and Salacia sp. grew after eight weeks, with a survival rate of 96% and 50%, respectively. The survival rate of F. tinctoria stem cutting after six weeks was 83.3%. These indicate that bajakah and akar kuning can be propagated and cultivated to support the bioprospecting of traditional medicinal plants. Despite some challenges, such as limitation of akar kuning and bajakah population at the pole stage in their habitats, it is an initial step of a long process of bajakah and akar kuning bioprospecting.
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