Pectin is one of the most important dietary fibers as a prebiotic to determine the composition of human gut microbiome. The study was designed to investigate the pectin degradation ability of Hominibacterium faecale strain SF3T, its isolated from human feces and cultivated with different concentration pectin under anaerobic conditions in vitro. The growth ratio of strain SF3T formed in batch culture was examined every 10 h during the 60-h incubation time using the spectrophotometer, high-performance liquid chromatography, and gas-chromatography. The pure culture of Hominibacterium faecale pectin degradation activity was present and commonly increases the growth value of strain SF3T after pectin fermentation. Regarding volatile fatty acids, acetate, propionate and butyrate levels rapidly raised after 30 h of incubation. The results suggest that pectin fermentation displays the greatest contribution for human health and confirm that pectin degradation leads to the production of acetate, butyrate and propionate.

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