The present study aims to prepare and And Characterization of zein nanofibers with simvastatin-loaded in it by electrospinning method using a polymeric matrix prepared from zein solution containing simvastatin antibiotics. The physical, cellular properties and drug release kinetics of the fibers were studied. Fiber formation was investigated by implementing a scanning electron microscope (SEM). The SEM images show a uniform network bead-free fiber structure created from the 1.5% simvastatin/zeinsolution. The viscosity of the 1.5% simvastatin/zein mixture increased to 0.402 P due to the presence of drug in zein solution. Also, the presence of the indicative peaks associated with simvastatin in the Fourier transform infrared (FTIR) spectra of the samples containing the drug, verifies the existence of the drug on the polymeric base of zein/polyethylene oxide (PEO). Also, around 53.887% of the loaded drug was released in the first 12 h of the release kinetics assay and the results of the MTT assay shows reduction in the number of live cells in the test sample. In conclusion, zein nanofibers containing simvastatin drug were found to be potential biodegradable polymers that can find application in bone tissue repair.