Transformer thermal modelling is a crucial aspect to be considered as this may help the determination of heat capacity of transformer. This paper present, simulation study on Oil Natural Air Natural (ONAN) transformer heat distribution pattern with and without presence of hot spot temperature (HST). This paper aims to compare the effects of different HST value at different locations inside the transformer unit as well as to evaluate the top oil thermal behaviour of corn oil as cooling mechanism in a transformer. To achieve aforementioned objectives, three HSTs were introduced to the 30 MVA transformer winding to find the total heat build-up in the top of the transformer tank. The outcome of thermal properties is examined using x-y temperature plot. From the results found that the location of HST affects overall transformer’s temperature. HST at the top of the winding give a significant effect compared to when HST is at the bottom of the winding. It is also evident that the usage of corn oil reduced the temperature distribution of the transformer. The findings suggest that the temperature distribution study especially on transformer is important to monitor in-service transformer in a non-invasive manner.

BackgroundTyrosine kinase inhibitors (TKIs) as first-line therapy for Chronic Myeloid Leukaemia (CML) show a high success rate. However, low number of patients with long-term treatment-free remission (TFR) is observed. Molecular relapse after imatinib discontinuation occurred at 50% at 24 months with 80% occurrence within the first 6 months. One of the reasons for relapse is untimely TKIs discontinuation caused by large errors from estimates at very low-level or undetectable disease, thus warranted new biomarkers for CML. MethodsNext Generation Sequencing (NGS) was used to identify microRNAs (miRNAs) at molecular response in CML adult patients receiving TKIs treatment. A total of 86 samples were collected, 30 from CML patients responsive and 28 from non-responsive to imatinib therapy, and 28 from blood donors. NGS was conducted whereby 18 miRNAs were selected and validated by real time RT-qPCR in triplicate. ResultsHsa-miR-181a-5p was expressed significantly (p-value<0.05) with 2.14 and 2.3 fold down-regulation in both patient groups respectively while hsa-miR-182-5p and hsa-miR-26a-5p were significant only in the non-responsive group with 2.08 and 2.39 fold up-regulation respectively. The down-regulation was consistent with decreased amount of BCR-ABL1 in patients taking TKIs regardless of molecular responses. The up-regulation was consistent with substantial presence of BCR-ABL1 in CML patients treated with TKIs at molecular response. ConclusionTherefore, these miRNAs have potential as new therapeutic biomarkers for BCR-ABL1 status in adult CML patients treated with TKIs at molecular responses. These could improve current approaches and require further analysis to look for targets of these miRNAs in CML.