Background After a decade of silence, an outbreak of the contagious and Asian endemic disease, goat pox re-emerged in North Vietnam affecting more than 1800 heads with a mortality rate of 6.5%. The inevitable impact of goat pox on hide quality, breeding, chevon and milk production has resulted in a significant economic losses to the developing goat industry of Vietnam. In the act of establishing an effective control of this devastating disease, tracing the source of re-emergence via a phylogenetic study was carried out to reveal their genetic relatedness. Either skin scab or papule from the six affected provinces were collected, cultured into Vero cells followed by restricted enzyme digestion of targeted P32 gene DNA encoding. The P32 gene was then cloned and transformed into E.coli competent cells for further sequencing. Results The isolated sequence is deposited into GenBank under Accession No. MN317561/VNUAGTP1. The phylogenetic tree revealed high similarity of nucleotide and amino acid sequences to references goat pox strains accounting for 99.6 and 99.3, respectively. The Vietnamese strain is clustered together with currently circulating goat pox virus in China, India and Pakistan which suggested the origin of South China. Conclusions This Vietnam isolate is clustered together with other Asian goat pox strains indicating the dissemination of a common goat pox virus within this continent.
| Aujeszky's disease (AD) is a common swine disease that widespread throughout the world. The symptoms include nervous signs, respiration and reproduction problems that lead to great economic losses to the industry. AD is endemic in Malaysia, where outbreaks had been reported in previous years. In Malaysia, approximately 95% of the pig farms are vaccinated for AD. Despite the regular vaccination, AD serological status remains unknown in this country. This study provides AD serological status in Peninsular Malaysia in 2016 based on the samples submitted to Faculty of Veterinary Medicine, University Putra Malaysia (UPM). A total of 1154 serum samples from 36 farms were submitted for AD ELISA diagnostic test; grouped as 8 weeks, 12 weeks, 16 weeks, 20 weeks, gilts and sows with different parity. The samples were subjected to AD antibody detection with IDEXX Pseudorabies Virus gpI Antibody Test Kit. Among the 36 farms submitted to UPM, 8 farms were detected with positive gI antibody indicated that these farms were still facing challenges from AD field virus. Among these eight seropositive farms, three farms were having seroprevalence in the range of 33.33% to 37.14%. In general, vaccination of AD is ideal and stable in Malaysia but we still need to be alert with the field challenge as it will be a threat to the industry.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.