This research studies the behaviour of hybrid nanofluid on mixed convection flow over a stretching sheet by considering the effect of non-uniform heat source/sink. The chosen base fluid is water with two different nanoparticles which are copper and aluminium oxide. The governing partial differential equations are reduced into ordinary differential equations using a similarity transformation technique. The resulting governing system then solved numerically using Keller-box method. The influence of space- and temperature-dependents of non-uniform heat source and sink parameters as well as the unsteadiness parameter is presented graphically. The current study shows that, the space- and temperature dependents of non-uniform heat source and sink significantly raises the fluid’s temperature. Comparative study also shows that hybrid nanofluid has higher temperature and heat transfer coefficient than single nanofluid and conventional fluid. It can also be concluded that, the enhancement of heat transfer rate can be achieved by reducing the parameter of space- and temperature dependents of non-uniform heat source and sink.