West Africa is one of the regions in Africa with the highest levels of malaria transmission a descriptive study was carried out to inventorise the medicinal plants traditionally used against malaria by traditional therapists in five malaria endemic areas of the Ségou region, in Mali. Sixty-five traditional therapists were randomly selected to be part of the study. Questions were posed using semi-structured interviews, which solicited information on species used, plant organs used, as well as methods of preparation and routes of administration of decoctions. Results indicate that 69 species distributed over 27 families are used to treat malaria. The most represented families are Fabaceae (24.63%), Combretaceae (13.04%), Rubiaceae (7.24%) and Meliaceae (5.79%). The most cited species are Argemone mexicana (CF=0.78), Combretum micranthum (CF=0.84), Conocarpus biocarpa (CF=0.70), Gardenia sokotensis (CF=0.75) and Mitragyna inermis (CF=0.81). The recipe mostly involves leaves (82.43%), with the decoctions mainly taken orally.