ObjectivesThe aim of the study was evaluation of the effectiveness of three different solvents (orange oil solvent, eucalyptol oil solvent, and chloroform) during retreatment regarding: root canal filling removal Methods were 33 premolar root canals were prepared by using Protaper universal files for cleaning and shaping, obturation was done by lateral compaction technique. Samples were divided (n=11) Group A Chloroform, Group B orange oil, and Group C Eucalyptus oil according to the solvent used into three groups. Retreatment was done by combining two methods for filling removal as chemical by using organic solvents and mechanical by utilizing rotary retreatment files (Protaper retreatment files). Roots were split longitudinally, canals were observed using a stereomicroscope at 6x. The images were subjected to morphometric analysis to assess the amount of remaining root filling materials. Data were explored for normality using Kolmogorov-Smirnov and Shapiro-Wilk tests, data showed parametric (normal) distribution. Repeated measure ANOVA was used to compare between more than two groups in related samples. Results:The highest mean value was found in (Group A) followed by (Group B), while the least mean value was found in (Group C). A statistically significant difference was found between (Group C) and each of (Group A) and (Group B) (p<0.001). No statistically significant difference was found between (Group A) and (Group B) (p=0.853). Conclusion:Chloroform and orange oil are preferable to be used in retreatment.