Lady finger (Abelmoschus esculentus (L.) Moench) pod contain polyphenolic compounds, carotene, folic acid, thiamine, riboflavin, niacin, vitamin C, oxalic acid, and amino acids. Lady finger seed contain polyphenolic compounds, mainly oligomeric catechins and flavonol derivatives, protein (i.e., high lysine levels) and oil fraction (in particular, its derived oil is rich in palmitic, oleic, and linoleic acids). This study aimed to formulate a sheet mask of Lady Finger extract, and examining its moisturizing effect on volunteers' skin. This was an experimental study, which conducted by maceration of Lady Finger simplicia, then formulated the essence into sheet mask sheet preparation. Lady Finger extract was varied in 3%, 5% and 7% concentration. Evaluation of preparation include of the quality of the preparation (organoleptic, homogeneity, pH value, and stability). Sheet mask was then examined for its irritation test and effectiveness as moisturizer on volunteers skin. Moisturizing effect was seen each week for 4 weeks treatment by using it twice a week. The results showed that all sheet masks were homogeneous, had pH of 5.6-6.1, stable for 12 days of storage by using cycling test method, and did not irritate the skin. Moisturizing effect of Lady Finger extract sheet mask at 7% concentration was more effective than other concentrations in terms of increasing the water content by 37.36% and smoothness by 40.02%. It can be concluded that Lady Finger (Abelmoschus esculentus (L.) Moench) extract can be formulated as an moisturizing sheet mask and 7% concentration of Lady Finger extract sheet mask preparation showed the best moisturizing activity. Keywords: Moisturizing, Sheet Mask, Lady Finger, Extract

Abstract Some diseases caused by microorganisms on the hands are diarrhea, diphteria, boils, typhus and others. The bacteria that most often contaminate the skin of the hands are Staphylococcus aureus and Escheria coli, whose spreas most often transmitted from hand to hand. Bunga lawing are one of the medicinal plants that have been used in medicine. The purpose of this study was determine the antibacterial activity of gel hand essential oil sanitizer gel preparation (Illiacium verum Hook.f) to the growth of Staphylococcus aureus and Escheria coli bacteria compared to sanitizer Dettol gel hand. This was carried out with the experimental method. Essential oil of bunga lawing is extracted using a heat method, namely soxhletation. The antibacterial activity test in this study used the agar diffusion method (disc diffusion). The measurement of the diameter of the inhibited area formed were analyzed using a one-way ANOVA statistical test. The results of antibacterial activity of bunga lawang sanitizer hand have lower antibacterial activity gel hand sanitizer Dettol. In the diameter of Staphylococcus aureus bacteria the highest rate 0f hand flower sanitizer gel was 15,1 mm while the sanitizer gel hand wash 21,4 mm. In Escheria coli bacteria, the inhibitory diameter the sanitizer hand gel dettol was obtained at 20.1 mm, while the lawang handsanitizer gel obtained the highest inhibition diameter of 13.1 mm. The results of data analysis using a one-way ANOVA statistical test showed a sig value p<0,05 so that there were significant differences between the gel hand to the inhibitory power of the growth of Staphylococcus aureus and Escheria coli bacteria.   Keywords: Bunga lawang, hand sanitizer gel, Staphylococcus aureus, Escherichia coli

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