Background: Probiotics and medicinal plants have been used to support human and livestock health. This research aimed to evaluate the potential of Moringa oleifera Lam. leaf extract on the growth of Pediococcus pentosaceus, Lactobacillus acidophilus and L. plantarum  probiotic in vitro and to prove its potential as a feed additive, as an alternative to antibiotic growth promoters (AGP) to improve nutrient intake, body weight gain, feed conversion ratio and feed efficiency in broiler chicken starter phase. Methods: This study consisted of three sub studies: (1) Screening test for phytochemical compounds; (2) evaluation of several doses of Moringa extract (0%, 0.1%, 0.2%, 0.3% ). At the end of treatment, each colony was measured using the total plate count; (3) evaluation of probiotics and M. oleifera in vivo to prove growth performance on starter-phase broiler chicken. All results were analyzed by analysis of variance (ANOVA) then followed by the Duncan test. Results: (1) The phytochemical screening test M. oleifera extract contained flavonoids, saponins, tannins, triterpenoids and alkaloids; (2) M. oleifera extract at doses of 0.1%, 0.2%, 0.3% increased the growth of bacteria Pediococcus pentosaceus, Lactobacillus acidophilus and Lactiplantibacillus plantarum  (p<0.05); (3) The use of probiotics, M. oleifera extract and their combination showed that body weight, body weight gain, feed conversion rate (FCR) and feed efficiency signifcantly differed (p <0.05) between the treatments, but there was no significant difference (p > 0.05) between the treatments on nutrient intake (dry matter, ash, crude protein, crude fiber and organic matter) on broiler chicken starter phase.  Conclusions: The use of M.oleifera extract at doses of 0.1%, 0.2% and 0.3% increased the growth of P. pentosaceus, L. acidophilus and L. plantarum bacteria in vitro and the use of probiotics, M.oleifera extract and their combination by in vivo improved the growth performance on starter phase of broilers chicken.

Background: Probiotics and medicinal plants have been used to support human and livestock health. This research aimed to evaluate the efficacy of Moringa oleifera Lam. leaf extract on the growth of P. pentosaceus, L.acidophilus and L. plantarum  during starter period on nutrient intake, body weight gain, FCR and feed efficiency in broiler chicken. Methods: This study consisted of three sub studies: (1) Screening test for phytochemical compounds. The flavonoid test was conducted by Bate Smith-Metcalf and Wilstatter method.  The Tannin test with Denis’ reagent.  The saponin test was performed by the Forth method.  Triterpenoid tests were performed by the Liebermann-Bouchard method and the alkaloids test was conducted by the method of Mayer, Bouchardat  and Wagner. (2) evaluation of level M.oleifera extract, where each test tube was added with 1 mL of each isolate and incubated at 37°C. The growth of probiotic bacteria was calculated by using the TPC. (3) evaluation of probiotics and M. oleifera in vivo to prove growth performance. All results were analyzed by analysis of variance (ANOVA) then followed by the Duncan test. Results: (1) The phytochemical screening test M. oleifera extract contained positive of flavonoids, saponins, tannins, triterpenoids and alkaloids; (2) in vitro evaluation of M. oleifera extract could increased the growth of bacteria probiotic (p<0.05); (3) The use of probiotics and M. oleifera extract could improve growth performance. The result of body weight, body weight gain, FCR and feed efficiency signifcantly differed (p <0.05), but there was no significant difference (p >0.05) on nutrient intake.  Conclusions: The use of M.oleifera extract at doses of 0.1%, 0.2% and 0.3% increased the growth of P. pentosaceus, L. acidophilus and L. plantarum bacteria in vitro and the use of probiotics, M.oleifera extract and their combination by in vivo improved the growth performance on starter phase of broilers chicken.

Background: Probiotics and medicinal plants have been used to support human and livestock health. This research aimed to evaluate the efficacy of Moringa oleifera Lam. leaf extract on the growth of P. pentosaceus, L.acidophilus and L. plantarum  during starter period on nutrient intake, body weight gain, FCR and feed efficiency in broiler chicken. Methods: This study consisted of three sub studies:(1) Screening test for phytochemical compounds. The flavonoid test was conducted by Bate Smith-Metcalf and Wilstatter method.  The Tannin test with Denis’ reagent.  The saponin test was performed by the Forth method.  Triterpenoid tests were performed by the Liebermann-Bouchard method and the alkaloids test was conducted by the method of Mayer, Bouchardat  and Wagner. (2) evaluation of level M.oleifera extract, where each test tube was added with 1 mL of each isolate and incubated at 37°C. The growth of probiotic bacteria was calculated by using the TPC. (3) evaluation of probiotics and M. oleifera in vivo to prove growth performance. All results were analyzed by analysis of variance (ANOVA) then followed by the Duncan test. Results: (1)The phytochemical screening test of M. Oleifera extract showed the presence of flavonoids, saponins, tannins, triterpenoids and alkaloids; (2) in vitro evaluation of M. oleifera extract could increased the growth of bacteria probiotic (p <0.05); (3) The use of probiotics and M. oleifera extract could improve growth performance. The result of body weight, body weight gain, FCR and feed efficiency signifcantly differed (p<0.05), but there was no significant difference (p >0.05) on nutrient intake.  Conclusions: The use of M.oleifera extract at doses of 0.1%, 0.2% and 0.3% increased the growth of P. pentosaceus, L. acidophilus and L. plantarum bacteria in vitro and the use of probiotics, M.oleifera extract and their combination by in vivo improved the growth performance on starter phase of broilers chicken.