ABSTRAKTujuan dari penelitian ini adalah mengetahui pengaruh penambahan kuning telur dalam pengencer CEP-2 terhadap motilitas dan viabilitas spermatozoa sapi Limousin. Penelitian menggunakan rancangan acak kelompok dengan 10 kali ulangan dari 10 ejakulat dari sapi yang berbeda. Spermatozoa disimpan selama delapan hari. Motilitas spermatozoa diamati dengan mikroskop cahaya (200x) pada suhu 37 C. Viabiltas spermatozoa diamati dengan pewarnaan eosin-negrosin menggunakan mikroskop cahaya (400x). Hasil penelitian menunjukkan suplementasi kuning telur pada pengencer CEP-2 berpengaruh terhadap motilitas dan viabilitas spermatozoa sapi Limousin selama penyimpanan pada suhu 4-5° C. Konsentrasi kuning telur terbaik adalah 20% dalam mempertahankan motilitas (44,25±3,92%) dan viabilitas (87,46±5,40%) spermatozoa sapi Limousin setelah penyimpanan delapan hari pada suhu 4-5° C. ____________________________________________________________________________________________________________________ Kata kunci: CEP-2, kuning telur, motilitas, viabilitas, spermatozoa sapi Limousin ABSTRACT The aim of this research was to study the effect of egg yolk supplementation in CEP-2 extender on motility and viability of Limousin bull sperm. The research used Randomized Block Design with ten repetitions from ten different bulls. Sperm was stored for eight days. Sperm motility was observed using light microscopy (200x) at temperature of 37 C. Sperm viability was observed with eosin negrosin staining by light microscopy (400x

Soya is an ingredient that is added to semen extender as a component of macromolecules. This study aims to examine the effect of adding soy in the Tris base diluent on the quality and integrity of the Senduro goat spermatozoa membrane. Spermatozoa membrane integrity was observed using the HOST method. The results showed the greatest motility, viability, and integrity of the spermatozoa membrane at the beginning of storage were found in the tris + soya diluent of 2%. But at the end of storage, ie on the second day after storage, the quality of spermatozoa in the control group and the treatment group (2% and 3%) did not differ only. Based on the results of the study it can be concluded that the use of soy in the tris base diluent has not been able to provide optimal protection in Spermatozoa Senduro Goat during storage at a temperature of 4-5⁰C.