The continued and increasing development of antimicrobial resistant bacteria among the foodborne pathogens had caused worldwide to be alarmed. Being the earliest to develop antimicrobial resistance, Staphylococcus aureus is constantly monitored for any new resistance development. The resistance development is often linked to wastewater and the treatment plants where the pressure of antibiotic is the highest. Hence, this study investigated on the prevalence of high antimicrobial resistant S. aureus in the wastewater eluted from a poultry slaughterhouse. A total of thirty wastewater samples were collected from a poultry slaughterhouse in Semenyih, Selangor. Most probable number (MPN)-plating method was employed to enumerate the S. aureus count in the wastewater. The results indicated that S. aureus was highly present whereby all samples (100%) were positive and the concentration ranged between 11 -2