DHF cases tend to increase from year to year in Indonesia. So the government makes efforts to control cases of Dengue Fever (DHF) which are usually done chemically and harm the environment and health. There is a need for safer, more effective, and environmentally friendly controls, such as using natural ingredients as natural larvicides. Among the natural ingredients having potential as a larvicide is Bintaro leaves (Cerbera manghas). The purpose of this study was to determine the toxicity of Bintaro leaf extract against third instar larvae of Aedes aegypti mosquitoes. This experimental study used 7 treatments of Bintaro leaf extract concentration (5 %, 15 %, 25 %, 35 %, 45 %, 65 %, 75 %), abate as a positive control, and distilled water as a negative control. Each treatment used ten instar III Aedes aegypti larvae with four repetitions. The data obtained were then analyzed using probit analysis to determine the toxicity of Bintaro leaf extract to Aedes aegypti larvae by calculating the LC50 and LC90 values. The results showed that the most effective concentration was 75 % because it could kill 100% of the test larvae. The LC50 value of 5,097 % and the LC90 value of 25,300 % indicate that the level of toxicity is very toxic. The probit regression analysis shows a linearity line equation y = 1.15 + 1.43x with a correlation (R2) of 0.512 which indicates that the correlation is strong enough. It is related to the content of flavonoids, tannins, saponins, triterpenoids which are toxic to the abdomen, nervous system, and respiratory system of larvae. From the research results, Bintaro extract with a concentration of 75 % can be used as a natural larvicide candidate. Furthermore, further research to see the toxicity to the environment can be done.are toxic to the abdomen, nervous system, and respiratory system of larvae. From the research results, Bintaro extract with a concentration of 75 % can be used as a natural larvicide candidate. Furthermore, further research to see the toxicity to the environment can be done.

Malang Regency is an endemic area for cases of DHF (Dengue Hemorrhagic Fever) in East Java Province. One of the efforts to reduce the number of cases is to control the breeding of the Aedes aegypti mosquito which is the main vector. However, this will depend on the susceptibility status of Aedes aegypti to the insecticides used, one of which is malathion. This study aims to determine the susceptibility status of Aedes aegypti mosquitoes to malathion insecticides in Malang Regency in the southern part. This descriptive observational study used adult Aedes aegypti mosquitoes obtained from the landing collection of larvae from three sub-districts in Malang Regency in the southern part (Kepanjen, Gondanglegi, and Bantur). The location selection was done by simple random sampling technique. The larvae of the Aedes aegypti mosquito were then reared in the Chemistry Laboratory, University of Muhammadiyah Malang. Adult mosquitoes with stomach conditions full of sugar were then tested using the susceptibility test which refers to WHO standards. The results showed that the mortality rate of the tested mosquitoes in the Districts of Kepanjen, Gondanglegi, and Bantur, respectively, reached 98%, 93%, and 96%. This shows that the Aedes aegypti mosquitoes in the three locations are classified as tolerant to the insecticide malathion by 0.8%. Thus, it can be concluded that the insecticide malathion is still effectively used as vector control for DHF (Dengue Hemorrhagic Fever) in Malang Regency.