This study was aimed to prepare polyeugenol with high molecular weight and to evaluate its antibacterial and antioxidant activities. First, polyeugenol was synthesized from eugenol in the presence of H2SO4-CH3COOH (4:1) as catalyst. The synthesized polyeugenol was weighed by using viscometer, revealing its high molecular weight of (7.76–21.9) × 105 g/mol. Furthermore, the antibacterial activity of the polyeugenol was conducted against Staphylococcus aureus and Escherichia coli bacteria. It was conducted by applying well diffusion method at 1, 2, 3, 4 and 5% concentrations to observe inhibition zones, in which the tests showed that the antibacterial activity of the polyeugenol against S. aureus were 17.42, 17.76, 18.79, 21.42 and 22.55 mm, while those against E. coli were 15.87, 17.23, 17.56, 18.24 and 19.21 mm, respectively. In short, these results indicated a strong antibacterial activity. Then, tests on antioxidant activity against free radical DPPH (2,2-diphenyl-1-pycrylhydrazyl) gave the IC50 value of 80.47 µg/mL, indicating a strong antioxidant activity. Therefore, the polymer synthesized in this work has a high potential to be applied in various biomedical applications.

Pineapple contains active compounds, such as phenolics, steroids, flavonoids, terpenoids, saponins, quinines, coumarins, alkaloids, and tannins. These compounds can be used as antioxidants, antibacterial, and antifungals. The purpose of this study was to determine the activity of compounds in pineapple steam in inhibiting the growth of Staphylococcus aureus, Shigella dysentry, Salmonella typhi in vitro and silico. The extraction method was carried out by maceration using 70% ethanol. Analysis of the compound in pineapple extract using LC-MS. In vitro antimicrobial activity test using the disc diffusion method. Toxtree software was used to test the toxicity of compounds. Physicochemical test of compounds using Lipinski Test. Docking molecular using PyRx software. Visualization of docking results using Discovery Studio 2019 Software. The results showed that the yield obtained from the extraction process of pineapple weevils was 79.3%. There were 58 compounds obtained from the LC-MS analysis. Compounds that have antibacterial activity are acetic acid, cinnamic acid, oxalic acid, malic acid, thiamine, niacin, riboflavin, p-coumaric acid, caffeic acid, ferulic acid, sinapic acid, quercetin, ananasic acid, subaphyllin, beta carotene, lutein, cyamin, cyaniding 3,5,3'-triglucoside, beta-sitosterol, campesterol, and hydroxysitosterol. Toxicity test results with toxtree software found that all compounds belong to Class I. Class, I compound that have low toxicity properties.