Stem cells play an essential role in maintaining homeostasis, as well as participating in new tissue regeneration. Over the past 20 years, a great deal of effort has been made to investigate the behaviour of stem cells to enable their potential use in regenerative medicine. However, a variety of biological characteristics are known to exist among the different types of stem cells due to variations in the methodological approach, formulation of cell culture medium, isolation protocol and cellular niches, as well as species variation. In recent years, cell-based therapy has emerged as one of the advanced techniques applied in both medical and clinical settings. Cell therapies aim to treat and repair the injury sites and replace the loss of tissues by stimulating the repair and regeneration process. In order to enable the use of stem cells in regenerative therapies, further characterisation of cell behaviour, in terms of their proliferation and differentiation capacity, mainly during the quiescent and inductive state is regarded as highly necessary. The central focus of regenerative medicine revolves around the use of human cells, including adult stem cells and induced pluripotent stem cells for cell-based therapy. The purpose of this review was to examine the existing body of literature on stem cell research conducted on cellular angiogenesis and migration, to investigate the validity of different strategies and variations of the cell type used. The information gathered within this review may then be shared with fellow researchers to assist in future research work, engaging in stem cell homing for cell-based therapy to enhance wound healing and tissue regeneration process.

Background: The migration and differentiation of stem cells take place during the reparative phase of the healing cascade. Chemokine ligands and receptors are the key players in the homing process dur-ing the early stage of capillary morphogenesis. Stem cells from exfo-liated deciduous teeth are known to possess a huge potential benefit for tissue regeneration. However, the gene expression of SHED en-gaging in angiogenesis and migratory activity during tissue healing is not fully understood. This study aims to assess the gene expression of SHED following in-vitro angiogenesis and migratory induction protocol. Methods: Scratch test assay was conducted following an angiogenic induction of SHED by supplementation of EGM-2 and VEGF. For the detection of migratory cell markers, angiogenic markers, and stem cell markers, RNA samples were extracted on day 1, 3, 7, 10, and 14 after the angiogenic induction in a transwell chamber, fol-lowed by RT-PCR analysis. Results: The findings suggested that SHED forming endothelial cells at higher capacity under an imma-ture state with higher seeding density. SHED undergoing angiogen-esis and migratory activity showed elevated IL-8, CCR1, CXCR4 and CCL28 expression. CCR1 expression significantly increased in the A+M+ group (p<0.05). Conclusion: The gene expression of these chemokines, particularly CCR1, which closely represent cellular migration, suggests the po-tential use of SHED for cell-based therapy to enhance tissue repair.

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