This is the first case report highlighting the application of an in-house autogenous vaccine for the treatment of bovine cutaneous papillomatosis Type 2 in a Jersey crossbred cow in Malaysia. A sample of papilloma growth was excised, homogenised, treated using 0.5% (v/v) formalin and performed microbiological examinations for sterility check. The solution was then administered subcutaneously to the cattle at seven days interval for four weeks. The papilloma growth on the affected cattle regressed rapidly following treatment, and there were no undesirable side effects and recurrences observed. The use of autogenous vaccine as an alternative treatment for bovine papillomatosis is preferred over surgical excision as it is proven effective, safe, and low chances of recurrence.