DNA Barcoding is recommended as a tool for identifying and confirming species within the taxonomy framework. The rbcL gene is the barcode DNA for plant species. Ternate Island is one of clove plantation center in North Maluku. The diversity and productivity of cloves on the Ternate island known since in earlier times. This study has a purpose to amplify the rbcL gene in clove plants collected from clove plantations of communities in Ternate island. Isolation of total DNA carried out with using ZymoBiomic (Zymo Research DNA Extraction) Kit from leaves tissue, then DNA specimen is amplified based on rbcL gene with forwarding sequence rbcLaF 5′-ATG TCA CCA CAA ACA GAG ACT AAA GC-3′ and reverse sequence is rbcLaR 5′-GTA AAA TCA AGT CCA CCR CG-3′. The results showed that the specimen was successfully amplified with an amplicon size of 600 bp. Furthermore, BLASTN analysis results note that the sequence has a similarity of 99% with Syzygium cumini vc. J.R. Abbott 23676 (FLAS) ribulose-1,5-bisphosphate carboxylase large subunit (rbcL) gene, but phylogenetically the precise position of sample could not found because the limitation of rbcL gene.
At present, coconut shell charcoal briquettes are one of the alternative energy sources that are in demand by the export market. The purpose of this research was to produce an alternative briquette product based on local natural materials. Kenari shell is a waste of natural materials or biomass. The characteristics of Kenari shells have the same structure with coconut shell. The pre-design of briquette production of this Kenari shell used pre-treatment process stages, charcoal making, destruction, printing with adhesive variation, drying, and SNI quality of briquette quality analysis. The results of pre-design of bio-briquette production of Kenari shell showed that the application of the method and the basic ingredients of waste could produce the product of charcoal briquettes equal to charcoal briquettes of coconut shell.
Tujuan: Penelitian ini bertujuan melihat best-practices implementasi lesson study dalam memfasilitasi pembelajaran yang berpusat pada peserta pada mata pelajaran IPS Ekonomi. Metode: Studi deskriptif kualitatif ini dilakukan di SMAN 10 Ternate selama bulan Agustus-November 2021 dengan melibatkan subyek sebanyak 36 siswa kelas X IPS1. Pengumpulan data menggunakan teknik observasi dengan menggunakan instrument berupa lembar observasi, rekaman proses pembelajaran, dan catatan harian peneliti yang kemudian dianalisis secara kualitatif. Temuan: hasil penelitian menunjukkan bahwa lesson study mampu memfasilitasi pembelajaran yang berpusat pada peserta didik melalui setiap implementasi pada tahapan designing, action, and reflection. Kesimpulan: Lesson study sebagai model peningkatan kompetensi guru melalui pengkajian praktik pembelajaran memberi kontribusi positif bagi guru dan peserta didik di kelas X IPS1 pada mata pelajaran Ekonomi. Lesson study memberi ruang kepada guru untuk mengembangkan kreatifitasnya dalam melakukan perubahan dan perbaikan. Sementara bagi peserta didik, lesson study memberi ruang hak belajar untuk difasilitasi mengembangkan potensi yang dimilikinya. Hasil penelitian ini menjadi rekomendasi bagi perbaikan kualitas pembelajaran di Sekolah Menengah Atas di Kota Ternate, khususnya pada mata pelajaran IPS.
The nutmeg extract had been recognized as the source of antioxidant and antimicrobial activity. The aims of this study were to obtain endophytic fungi isolated from the mace and seed of Nutmeg (Myristica fragrans Houtt) and to determine antifungal activity of those isolates. Microbiol routine assay were conducted and it consists of three stages, namely isolation of endophytic fungi, identification of fungus and antifungal activity test by using dual culture. Sixteen endophytic fungus isolates were found in this study. Those are identified as Alternaria sp (BPB 1.
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