Bacterial contamination issues are commanding the attention of water and wastewater treatments. There are many treatment methods to eliminate pathogenic bacteria. Therefore, finding efficient disinfection methods is essential to achieving safe water and protecting the human health. Therefore, this paper aims to isolate and identify Escherichia coli from municipal wastewater treatment plant and to investigate the performance of flow cell ultrasound as the bacterial disruption. In order to confirm the identification of Escherichia coli isolated, 16S rRNA sequence analysis was involved. In this particular study, the performance of flow cell ultrasound was measured by disruption of Escherichia coli cells concentration (10 4 cfu/ml), two types of flow rate (25 and 70 ml/min) with varies amplitude and power intensity. Escherichia coli isolated was determined and high homology was found that 16 s rRNA sequence of Escherichia coli with a level of identity 95 %. The flow rate at less power intensity and less oscillations amplitude at 35 % is statistically significant and higher of bacterial disruption than the greater power intensity, p = 001. Meanwhile, higher flow rate was affected by increases of cycle treatment and consequences of the higher disruption of Escherichia coli cells. In addition, ultrasound technology, which includes as an advance technology as improvements to the sustainable technology associated with the increases of public health and environmental quality.
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