With the rapid development of our country’s economy, there has been a phenomenon of population concentration and centralization of medical institutions. At the same time, with the continuous development and improvement of medical disciplines, large-scale general hospitals have become more comprehensive and larger in area. Therefore, hospital users often get lost in the public space of the hospital. Therefore, the purpose of this article is to study the evaluation method of wayfinding signs in medical institutions based on mobile network intelligent navigation. This article first analyzes the research status of navigation signs in medical institutions and then introduces the current wayfinding behaviors of large general hospitals, and analyzes the problems and deficiencies in them. On this basis, the intelligent navigation is researched and designed. This article systematically expounds the main characteristics of hospital users’ pathfinding behavior, the spatial order of the process, and the hierarchy of pathfinding behavior, and uses questionnaire survey, field survey, and other research forms to carry out experimental research on the theme of this article. Studies have shown that the hospital’s public organization space model will directly affect the efficiency of people seeking medical care.