To complete this review, relevant literatures were collected from several scientific databases, include Google Scholar, Pubmed and ScienceDirect between 2000 to till date. The categories of keywords used were "Lutein" and "Sources" or "Pharmacological Studies" or "Molecular Mechanism" or "Biological Properties" or "Chemistry" or "Food Industry" or "Food Products" or "Formulations" or "Health Benefits". Studies which are not written in English and have no abstracts have been excluded from initial screening. There was no restriction to be followed for collecting the lutein-related studies, particularly in animal and human studies. After applying the inclusion and exclusion criteria and removing duplicates from the ABSTRACT Background: Lutein is a naturally occurring carotenoid found in high amounts in flowers, grains, fruits and green vegetables with green leaves include spinach, kale and carrots. The market for lutein encompasses pharmaceutical, dietary supplement, food, animal and fish feed industries. Objective: The present review aimed to provide an updated and comprehensive analysis of lutein, including its chemistry, biological properties and therapeutic potentials. Methods: Relevant literatures were collected from several scientific databases, include Google Scholar, Pubmed and ScienceDirect between 2000 to till date. Following a detailed inclusion and exclusion screening process, the information obtained was summarized. Results: Information on the sources, chemistry and biological properties including antioxidant, anti-arthrisits, antiinflammatory, hepatoprotective, cardioprotective, anti-cataract, antidiabetic, anticancer and bone remodelling activities, as well as food industry processing for lutein were tabled. Lutein can be considered powerful antioxidants along with multifaceted molecular targets, such as NF-ҡB, PI3K/Akt, Nrf-2, HO-1 and SIRT-1 signaling pathways in various pathological conditions. Conclusion: The present review observe the chemical, pharmacological properties, in addition to the therapeutic potentials of lutein. It is hoped that the information can provide a good reference to aid in the development and utilization of lutein in phytopharmaceuticals and food industries.