In this study, bacterial cellulose (BC) was produced by Acetobacter xylinum using oil palm frond (OPF) juice as a carbon source, where the effect of cultivation time in the range of 2-14 days on BC production was investigated. The highest BC production of 0.3365 g/L was obtained at cultivation time of 9 days. Structural analysis by FTIR showed that the properties of BC to be similar regardless of cultivation time. FESEM analysis revealed that the BC produced was composed of randomly packed and loosely fine cellulose fibers. The present study showed the feasibility of using OPF juice as an alternative in the production of BC which could reduce the production cost of BC.

The development of alternative food packaging films using bio-based residues is in great demand for replacing petroleum-based packaging materials. However, large-scale application is severely limited by costly production and poor performance. This study investigates the ex-situ modification of bacterial cellulose (BC) produced by Acetobacter xylinum in oil palm fronds juice to obtain BC-Chitosan (BCC) films. FTIR revealed the structure of amide I and II bands, confirming the presence of chitosan in BCC films. The FE-SEM images of BCC films showed the formation of a thick chitosan layer with increasing chitosan incorporated into the BC surface structure. The coated chitosan layer observed improved mechanical properties in BCC films due to the disappearance of empty pores between BC fibers. Increments in chitosan concentration slightly decreased the thermal behavior of BCC. The antimicrobial effects of BCC films were effective against Gram-positive bacteria (Staphylococcus aureus) when the concentration of chitosan incorporated was above 0.6 %w/v. This study reveals the potential of extending the application of BC derived from oil palm frond juice (OPFJ) for developing food packaging materials.