The use of whitening vitamin C to make the skin brighter in the long term, however can cause side effects. The purpose of this study was to investigate the side effects of long-term high dose injections of whitening vitamin C plasma glucose and cholesterol level. This study used rats (Rattus norvegicus L.) as experimental animals. The rats randomly divided into five groups consisting of ten rats each group. Four groups were administered injections of white vitamin C and one group as control animal. The design used in this study was a complete randomized design (RAL) and the treatments were different long term of injections of high-dose whitening vitamin C, ie 30-day injections (P1), 50-day injections (P2), 70-day injections (P3), 90 days of injections (P4) and control (P0). The treatment was repeated ten times. The parameters observed were glucose and cholesterol rats plasma levels before and after high-dose whitening vitamin C injection treatment. Data was analysed by ANOVA allowed by Duncan’s Test. The results indicated that rats glucose plasma levels injected by high dose of whitening vitamin C in long-term between control and treatment showed significant differences (P = 0.005) and cholesterol plasma levels showed significant different too (P = 000). Glucose and cholesterol plasma levels of rats injected by high doses of whitening vitamin C in long term tend to decrease.